An Analysis of the Mechanism About CO Enrichment Promoting Carbohydrate Metabolism in Cucumber ( L.) Leaves.

Elevated CO can affect the synthesis and distribution of photosynthetic assimilates. However, the carbohydrate metabolism molecular mechanism of cucumber leaves in response to CO enrichment is unclear. Therefore, it is of great significance to investigate the key functional regulatory genes in cucumber.

Redefining intestinal stemness: The emergence of a new ISC population.

In tissue homeostasis, intestinal stem cells (ISCs) undergo continuous self-renewal to sustain rapid cellular turnover. In this issue of Cell, Capdevila et al. and Malagola, Vasciaveo, et al.

A Coculture System for Modeling Intestinal Epithelial-Fibroblast Crosstalk.

Epithelial organoid monoculture is a powerful tool to model stem cell dynamics in vitro. However, extensive efforts have recently revealed various niche players and their significant roles in regulating epithelial stem cells. Among these niche components, fibroblasts have been heavily recognized in the field as a critical niche signal secretor.

Transcriptome Sequencing Reveals the Mechanism of Auxin Regulation during Root Expansion in Carrot.

Carrot is an important vegetable with roots as the edible organ. A complex regulatory network controls root growth, in which auxin is one of the key players. To clarify the molecular mechanism on auxin regulating carrot root expansion, the growth process and the indole-3-acetic acid (IAA) content in the roots were measured in this experiment.

Shift in prevalence and systemic inflammation levels from NAFLD to MAFLD: a population-based cross-sectional study.

Background: Variations in the prevalence and systemic inflammatory (SI) status between non-alcoholic fatty liver disease (NAFLD) and newly defined metabolic dysfunction-associated fatty liver disease (MAFLD) have only been reported by few studies. Hence, this study aimed to compile data on the prevalence and the systemic inflammation levels of MAFLD and NAFLD in a general population from Southeast China was summarized to explore the potential effect of the transformation of disease definition.

Methods: A total of 6718 general population participants aged 35-75 were enrolled.

Polymyxin B and fusidic acid, a novel potent synergistic combination against and isolates with polymyxin B resistance.

The increasing prevalence of multidrug-resistant (MDR) Gram-negative bacteria and comparatively limited options of antibiotics pose a major threat to public health worldwide. Polymyxin B is the last resort against extensively resistant Gram-negative bacterial infections. However, a large number of Gram-negative bacteria exhibited high-level resistance to Polymyxin B, bringing challenges for antimicrobial chemotherapy.

Molecular Regulatory Network of Anthocyanin Accumulation in Black Radish Skin as Revealed by Transcriptome and Metabonome Analysis.

To understand the coloring mechanism in black radish, the integrated metabolome and transcriptome analyses of root skin from a black recombinant inbred line (RIL 1901) and a white RIL (RIL 1911) were carried out. A total of 172 flavonoids were detected, and the analysis results revealed that there were 12 flavonoid metabolites in radish root skin, including flavonols, flavones, and anthocyanins. The relative concentrations of most flavonoids in RIL 1901 were higher than those in RIL 1911.

Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls.

Melatonin, a multi-regulatory molecule, stimulates root generation and regulates many aspects of plant growth and developmental processes. To gain insight into the effects of melatonin on adventitious root (AR) formation, we use cucumber seedings subjected to one of three treatments: EW (hypocotyl exposed and irrigated with water), SW (hypocotyl shaded and irrigated with water) and SM (hypocotyl shaded and irrigated with 100 µM melatonin). Under shaded conditions, melatonin induced significant AR formation in the hypocotyl.

Preparation and Application of a Magnetic Oxidized Micro/Mesoporous Carbon with Efficient Adsorption for Cu(II) and Pb(II).

Water pollution is a worldwide problem that requires urgent attention and prevention and exceeding use of heavy-metal ions is one of the most harmful factors, which poses a serious threat to human health and the ecological environment. In this work, a magnetic oxidized micro/mesoporous carbon (MOMMC) was prepared for the easy separation of Cu(II) and Pb(II) from water. The dual-template method was used to prepare micro/mesoporous carbon using sucrose as the carbon source, silica nanoparticles formed by tetraethyl orthosilicate as the microporous templates, and triblock copolymer F127 as the mesoporous template.

Modeling the role of emotion regulation and critical thinking in immunity in higher education.

It is deemed that the effectiveness of teachers is highly entangled with psycho-emotional constructs, such as critical thinking (CT), emotion regulation (ER), and immunity. Despite the potential roles of CR, ER, and immunity, their possible relationships have remained unexplored in the higher education context of Iran. To fill in this lacuna, this study explored the potential role of CT and ER in university teachers' immunity in the Iranian higher education context.

Multifunctional Silica-Based Amphiphilic Block Copolymer Hybrid for Cu(II) and Sodium Oleate Adsorption in Beneficiation Wastewater.

Beneficiation wastewater contains various types of pollutants, such as heavy metal ions and organic pollutants. In this work, a silica-based amphiphilic block copolymer, SiO-g-PBMA-b-PDMAEMA, was obtained by surface-initiated atom transfer radical polymerization (SI-ATRP) for Cu(II) and sodium oleate adsorption in beneficiation wastewater, using butyl methacrylate (BMA) as a hydrophobic monomer and 2-(dimethylamino)ethylmethacrylate (DMAEMA) as a hydrophilic monomer. FTIR, TGA, NMR, GPC, XRD, N adsorption-desorption isotherms and TEM were used to characterize the structure and morphology of the hybrid adsorbent.

Integrating physiology, genetics, and transcriptome to decipher a new thermo-sensitive and light-sensitive virescent leaf gene mutant in cucumber.

Chloroplasts are the material basis of photosynthesis, and temperature and light severely affect chloroplast development and thus influence photosynthetic efficiency. This study identified a spontaneous virescent leaf mutant, SC311Y, whose cotyledons and true leaves were yellow and gradually turned green. However, temperature and light affected the process of turning green.

Simultaneous Removal of Cr(VI) and Phenol from Water Using Silica-di-Block Polymer Hybrids: Adsorption Kinetics and Thermodynamics.

Heavy metal ions and organic pollutants often coexist in industrial effluents. In this work, silica-di-block polymer hybrids (SiO-g-PBA-b-PDMAEMA) with two ratios (SiO/BA/DMAEMA = 1/50/250 and 1/60/240) were designed and prepared for the simultaneous removal of Cr(VI) and phenol via a surface-initiated atom-transfer radical polymerization process using butyl methacrylate (BA) as a hydrophobic monomer and 2-(Dimethylamino)ethylmethacrylate (DMAEMA) as a hydrophilic monomer. The removal efficiency of Cr(VI) and phenol by the hybrids reached 88.

Role and mechanism of circular RNA circ_0050486 in regulating oxidized low-density lipoprotein-induced injury in endothelial cells.

Background: Dysfunction of endothelial cells in the arterial vasculature is an essential contributor to the pathogenesis of atherosclerosis. Circular RNAs (circRNAs) exert important regulatory functions in endothelial cell dysfunction. Here, we explored the precise role and mechanism of circ_0050486 in regulating endothelial cell injury induced by oxidized low-density lipoprotein (ox-LDL).

Combined analysis of carotenoid metabolites and the transcriptome to reveal the molecular mechanism underlying fruit colouration in zucchini ( L.).

To reveal the molecular mechanism underlying peel colouration, carotenoid metabolites and the transcriptome were jointly analysed in zucchini peels with three different colours: light green (Lg), yellow (Y), and orange (O). Our results showed that the carotenoid levels in O (157.075 μg/g) and Y (22.

Development of a scalable method to isolate subsets of stem cell-derived pancreatic islet cells.

Cell replacement therapy using β cells derived from stem cells is a promising alternative to conventional diabetes treatment options. Although current differentiation methods produce glucose-responsive β cells, they can also yield populations of undesired endocrine progenitors and other proliferating cell types that might interfere with long-term islet function and safety of transplanted cells. Here, we describe the generation of an array of monoclonal antibodies against cell surface markers that selectively label stem cell-derived islet cells.

Analysis of Glucosinolate Content and Metabolism Related Genes in Different Parts of Chinese Flowering Cabbage.

Glucosinolates (GSLs) are important secondary metabolites that play important defensive roles in cruciferous plants. Chinese flowering cabbage, one of the most common vegetable crops, is rich in GSLs and thus has the potential to reduce the risk of cancer in humans. Many genes that are involved in GSL biosynthesis and metabolism have been identified in the model plant ; however, few studies investigated the genes related to GSL biosynthesis and metabolism in Chinese flowering cabbage.

Clinical and Microbiological Characteristics of Mycobacterium kansasii Pulmonary Infections in China.

Mycobacterium kansasii, an important opportunistic pathogen of humans, causes serious pulmonary disease. Sixty M. kansasii isolates were collected for investigating the clinical characteristics of patients with M.

The Prevalence and Determinants of Fusidic Acid Resistance Among Methicillin-Resistant Clinical Isolates in China.

The significant increase in resistance of methicillin-resistant (MRSA) to fusidic acid (FA) is a worrying public concern. However, the data on the prevalence of FA-resistant MRSA isolates in China is still limited. This study aims to investigate the prevalence of FA resistance and resistance determinants among MRSA isolates from six tertiary hospitals in different regions of China between 2016 and 2020.

Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of Gene Mutations in Methicillin-Resistant .

Introduction: Methicillin-resistant (MRSA) infections have become a leading cause of severe infections in both healthcare and community settings. Mutations in the gene cause resistance to rifampin (RIF), a critical antibiotic for the treatment of multidrug-resistant . The aim of this study was to detect the molecular characteristics of RIF MRSA and analyze the gene mutations involved in RIF resistance.

Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells.

Despite numerous efforts to generate vascular tissues that recapitulate the physiological characteristics of native vessels, vascular cell source remains one of the principal challenges in the construction of tissue-engineered vascular grafts (TEVGs). Human pluripotent stem cells, therefore, represent an indispensable source to supply a large production of vascular smooth muscle cells (VSMCs) for cell-based therapy. In particular, human induced pluripotent stem cells (hiPSCs) generated from the same individual have opened up new avenues of achieving patient specificity through the derivation of autologous and immunocompatible VSMCs.

Circ_0010283/miR-377-3p/Cyclin D1 Axis Is Associated With Proliferation, Apoptosis, Migration, and Inflammation of Oxidized Low-density Lipoprotein-Stimulated Vascular Smooth Muscle Cells.

Circular RNAs have been reported as vital regulators and promising therapeutic targets in multiple human diseases, including atherosclerosis (AS). However, the functional roles of circ_0010283 in AS remain unclear. The real-time quantitative polymerase chain reaction was used to determine the expression levels of circ_0010283, microRNA (miR)-377-3p, and cyclin D1 (CCND1) in serum samples.

The genes crucial to carotenoid metabolism under elevated CO levels in carrot (Daucus carota L.).

The CO saturation point can reach as high as 1819 μmol· mol in carrot (Daucus carota L.). In recent years, carrot has been cultivated in out-of-season greenhouses, but the molecular mechanism of CO enrichment has been ignored, and this is a missed opportunity to gain a comprehensive understanding of this important process.