Publications by authors named "Meike Kloster"
Article Synopsis
- A novel multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique uses a Spatial Light Modulator (SLM) with an Electron Multiplying-CCD (EM-CCD) camera to generate multiple laser spots and effectively capture fluorescent signals.
- The SLM creates a phase map that allows for the production of diffraction-limited spots, achieving impressive time resolution of 100 microseconds, while maintaining observation volumes comparable to traditional confocal setups.
- Experiments on mouse embryonic fibroblasts and H1299 cells revealed insights into G-actin diffusion dynamics and changes in HSF1 molecule behavior during heat shock, demonstrating the technique's utility in studying cellular processes.
We report the first homogeneous sandwich immunoassay with gold nanoparticles (AuNPs) as fluorescence quenchers. The sandwich assay is designed for the detection of the protein cardiac troponin T (cTnT) by its simultaneous interaction with two different antibodies, one attached to AuNPs and the other labeled with fluorescent dyes. We demonstrate the working principle of the assay and using time-resolved fluorescence spectroscopy, we determine the quenching efficiency of the gold nanoparticles.
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