The impact of age and number of mutations on the size of clonal hematopoiesis.

Clonal hematopoiesis (CH) represents the clonal expansion of hematopoietic stem cells and their progeny driven by somatic mutations. Accurate risk assessment of CH is critical for disease prevention and clinical decision-making. The size of CH has been showed to associate with higher disease risk, yet, factors influencing the size of CH are unknown.

Clinical efficacy of vibration stimulation therapy to relieve acute exercise fatigue.

Background: Previous studies showed that local vibration stimulation therapy was effective in relieving fatigue, and the effects of different modes of vibration stimulation therapy were further investigated.

Objective: This study aimed to examine the effects of different vibration stimulation modes on relieving acute exercise fatigue based on the multiphysiological indicators such as heart rate variability (HRV), skin conductance level (SCL), and ratings of perceived exertion (RPE) subjective scale.

Methods: Sixty participants selected from the dragon boat team of the Shanghai University of Traditional Chinese Medicine were divided into acupoint stimulation group (20 participants), muscle stimulation group (20 participants), and control group (20 participants) by complete randomization.

Clinical studies on the electric automatic massage therapy for recovery of acute sports fatigue.

Background: Acute sports fatigue impairs athletes' performance and causes other health issues; therefore, an effective method of relieving acute sports fatigue is being researched.

Objective: The present study was envisaged to evaluate the effect of electric auto-massage therapy and proprioceptive neuromuscular facilitated (PNF) stretching method on the recovery of acute exercise fatigue using the heart rate variability-based multi-physiological index and RPE scale, and to explore the underlying principle and mechanism.

Method: Sixty volunteers were divided into the stretching group, massage group and control group (20 subjects each) using the complete randomization method.

Response Surface Optimized Infrared-Assisted Extraction and UHPLC Determination of Flavonoid Types from Flos Sophorae.

Single-factor experiment and Box-Behnken design were applied to optimize the infrared-assisted extraction (IRAE) of rutin, quercetin, kaempferol, and isorhamnetin from Flos sophorae. Four factors (extract solvent, solid-liquid ration, extraction time, infrared power) affecting the extraction yield of flavonoids were studied. Under optimized conditions the extraction yield was 33.

Application of near-infrared spectroscopy for the rapid quality assessment of Radix Paeoniae Rubra.

Near-infrared (NIR) spectroscopy with multivariate analysis was used to quantify gallic acid, catechin, albiflorin, and paeoniflorin in Radix Paeoniae Rubra, and the feasibility to classify the samples originating from different areas was investigated. A new high-performance liquid chromatography method was developed and validated to analyze gallic acid, catechin, albiflorin, and paeoniflorin in Radix Paeoniae Rubra as the reference. Partial least squares (PLS), principal component regression (PCR), and stepwise multivariate linear regression (SMLR) were performed to calibrate the regression model.

Community Physician-Guided Long-Term Domiciliary Oxygen Therapy Combined With Conventional Therapy in Stage IV COPD Patients.

Purpose: The aim of the study was to explore clinical effect of community physician-guided long-term domiciliary oxygen therapy (LTDOT) on patients with Stage IV chronic obstructive pulmonary disease (COPD).

Design: A retrospective study.

Methods: Fifty-four patients with Stage IV COPD were recruited and randomly divided into two groups (the LTDOT group and the control group).

[Analysis of dissolution characteristics and content of metal elements in Scutellaria baicalensis].

ICP-OES analytical technique was used to determine the contents of Ca, Mg, Cu, Zn, Fe and Mn in S. baicalensis from different origins, and the dissolution characteristics and the contents of 6 elements were analyzed. There were significant differences in the contents among the prepared medical materials (p < 0.

An effective solution to discover synergistic drugs for anti-cerebral ischemia from traditional Chinese medicinal formulae.

Recently, the pharmaceutical industry has shifted to pursuing combination therapies that comprise more than one active ingredient. Interestingly, combination therapies have been used for more than 2500 years in traditional Chinese medicine (TCM). Understanding optimal proportions and synergistic mechanisms of multi-component drugs are critical for developing novel strategies to combat complex diseases.

Two new C-methyl flavanones from the rhizomes and frond bases of Matteuccia struthiopteris.

Two new C-methyl flavanones, (2S)-5,7-dihydroxy-6,8-dimethyl-4'-methoxydihydroflavone-7-O-(6″-O-acetyl)-β-d-glucopyranoside (1) and (2S)-5,7-dihydroxy-6,8-dimethyldihydroflavone-7-O-(6″-O-acetyl)-β-d-glucopyranoside (2), together with five known compounds, demethoxymatteucinol-7-O-β-d-glucopyranoside (3), matteucinol-7-O-β-d-glucopyranoside (4), 5,7-dihydroxy-6-methyl-4'-methoxydihydroflavone (5), methoxymatteucin (6), and thunberginol C (7), were first isolated from the EtOH extract of the rhizomes and frond bases of Matteuccia struthiopteris. The structures were established by spectral analyses, mainly HR-ESI-MS and 1D and 2D NMR experiments (COSY, HSQC, and HMBC).

By improving regional cortical blood flow, attenuating mitochondrial dysfunction and sequential apoptosis galangin acts as a potential neuroprotective agent after acute ischemic stroke.

Ischemic stroke is a devastating disease with a complex pathophysiology. Galangin is a natural flavonoid isolated from the rhizome of Alpina officinarum Hance, which has been widely used as an antioxidant agent. However, its effects against ischemic stroke have not been reported and its related neuroprotective mechanism has not really been explored.

[Determination of 5-hydroxy-7-methoxyflavan in Daemonorops draco by HPLC].

Objective: To establish a method for the determination of 5-hydroxy-7-methoxyflavan in Daemrnonorops draco by HPLC.

Method: Dikma Diamonsil C15 column(4. 6 mm x 150 mm, 5 microm) was used.

[Pharmacological research of wild and cultivated Atractylodes lancea].

Objective: To explore the differences of the main pharmacological action in wild and cultivated Atractylodes lancea.

Method: Study the effect of the 70% ethanol extracts from the wild and the culticated A. lancea on the mice with gastriculcer induced by absolute ethyl alcohol or HCl.

[Advances in chemistry, molecular biology and pharmacological of Cangzhu].

We described chemical composition in Cangzhu in recent years, volatile oil is the important chemical composition, The beta-eudesmol, hinesol are active ingredient in volatile oil and there are 38 kind of glycosides. At the same time, we overview the applying of RAPD technology in atractylodes lancea. The results is that there are correlation in chemical composition, genetic differentiation and geographical distribution, there is some truth in bounded by a territorial division of the north-south Cangzhu, and genetic differentiation has been happened in atractylodes lancea to adapting the environment.

[Quality evaluation of Flos Carthami].

Objective: To develop methods for qualitative and quantitative analyses of Flos Cartnami from three aspects, pigments, flavonoids and adenosine.

Method: A method using HPLC coupled with electrochemical detector was developed to determine the content of hydroxysafflor yellow A and fingerprint of Flos Carthami. The chromatographic separation was performed on a Zorbax SB C18 column (4.

[Studies on chemical constituents of rhizome of Matteuccia struthiopteris].

Objective: To study the chemical constituents of the rhizome of Matteuccia struthiopteris.

Method: The constituents were separated and purified by column chromatography with silica gel and Sephadex LH-20. Their structures were identified on the basis of physical and spectral data.

[Determination of five primary chemical constituents in Cassia angustifolia by HPLC].

Objective: To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.

Method: The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.

[Chemical constituents from the leaves of Cassia angustifolia].

Objective: To study the chemical constituents from the leaves of Cassia angustifolia.

Methods: Compounds were isolated and repeatedly purified by chromatographic techniques on silica gel column. Their structures were elucidated by chemical and spectral methods.

[Experimental studies on hepatotoxicity of rats induced by Fructus Gardeniae].

Objective: To study the hepatotoxicity effects in rats with different extract of Fructus Gardeniae.

Method: Observe the change of appearance, behavior and weight of rats through oral gavage daily for 3 d. Weigh the liver and calculate the liver index.

[Determining the quantity of hypericin in medicinal materials and asepsis seedings of Hypericum perforatum by HPLC].

Objective: To establish the method of determining the quantity of hypericin in Hypericum perforatum and determine the quantity of the hypericin in defferent medicinal materials and asepsis seedings which grow in defferent environment.

Method: The specimen is extracted with methanol--Pyridine (9:1) ultrasound extraction. Chromatographic assay is performed on a hypersily ODS2 (4.