A potential role for CPY17 as a parameter in differentiation between aldosterone-producing adenoma and nodular hyperplasia in patients with hyperaldosteronism.

Objective: To investigate CYP17 mRNA and protein expressions in aldosterone-producing adenoma (APA), nodular hyperplasia (NH) and normal adrenal gland (NAG) and if CPY17 might be used as a potential marker to differentiate between APA and NH in patients with hyperaldosteronism.

Methods: Total RNA and protein were extracted from APA, 12 NH, and 15 NAG tissues. mRNA and protein expressions of CPY17 were examined by real-time polymerase chain reaction (PCR) and Western blot analysis.

Expression of P-450(c11beta) in adrenal aldosterone-producing adenomas and nodular hyperplasia tissues.

Background: Our previous study suggests that decreased P-450(c17alpha) expression correlated with the overproduction of aldosterone in APA and nodular hyperplasia in patients with primary aldosteronism. This study was performed to further investigate if P-450(c11beta) contributes to the overproduction of aldosterone in APA and nodular hyperplasia tissues.

Methods: Total RNA and protein were extracted from 7 cases of APA tissue, 3 nodular hyperplasia tissues, 7 normal adrenal glands.

A dispensable role for P450(scc) in the overproduction of aldosterone in aldosterone-producing adenoma and idiopathic hyperaldosteronism in patients with primary aldosteronism.

Our previous study suggests that cytochrome P-450 carbon 17α-hydroxylase/17,20-lyase (P450(c17α)) correlated with the overproduction of aldosterone in aldosterone-producing adenoma (APA) and idiopathic hyperaldosteronism (IHA) in patients with primary aldosteronism. To further investigate if cytochrome P-450 cholesterol side-chain cleavage enzyme (P450(scc)) contributes to the overproduction of aldosterone in APA and IHA and if its mRNA expression differs in APA and IHA in patients with primary aldosteronism, we studied the expression of P450(scc) mRNA in APA and idiopathic hyperplastic nodules. Total RNA was extracted from APA of eight patients diagnosed as APA, idiopathic hyperplastic nodules of four patients diagnosed as IHA, seven normal adrenal glands and one normal muscle tissue.

[Clinic analysis of repeat prostatectomy after transurethral prostatectomy for benign prostatic hyperplasia].

Objective: To analyze the incidence rate of repeat transurethral prostatectomy (TURP) and its probable causes after TURP for benign prostatic hyperplasia (BHP).

Methods: A retrospective study was conducted to summarize the cases who received repeat transurethral resection of prostate after TURP for benign prostatic hyperplasia in recent 20 years. The data were compared with the data of the cases who just received once TURP for BPH.

[Telomerase expression in various lesions of adrenal cortex].

Objective: To investigate the expression of telomerase in various lesions of adrenal cortex.

Methods: By autoradiography-based telomeric repeat amplification protocol, telomerase expression was detected in 36 samples of adrenocortical lesions, including 29 cases adrenocortical adenoma (8 Cushing's syndrome, 17 aldosteronism and 4 nonfunctional adenomas), 5 cases of hyperplasia of adrenal cortex (presented with Chushing' syndrome), 2 cases adrenocortical carcinoma, and 4 samples of normal adrenal cortex.

Results: Of the 40 samples, 2 cases of adrenocortical carcinomas had telomerase expression, and the others had no telomerase expression detected.