Development of a rapid detection method for enrofloxacin in food.

Develop the ic-ELISA rapid detection method of Enrofloxacin (ENR). Corresponding antibodies are obtained by animal immunity to identify their titer and specificity. The optimal coating time was obtained by indirect competition ELISA, and the antigen coating time, suitable coating concentration, primary antibody dilution factor, blocking solution blocking time, primary antibody reaction time and secondary antibody reaction time were optimized, and the specificity and accuracy of the method were evaluated.