Dynamic light scattering microscopy sensing mitochondria dynamics for label-free detection of triple-negative breast cancer enhanced by deep learning.

We established a deep learning-based dynamic light scattering (DLS) microscopy sensing mitochondria dynamic for label-free identification of triple-negative breast cancer (TNBC) cells. The capacity of DLS microscopy to detect the intracellular motility of subcellular scatters was verified with the analysis of the autocorrelation function. We also conducted an in-depth examination of the impact of mitochondrial dynamics on DLS within TNBC cells, employing confocal fluorescent imaging to visualize the morphology of the mitochondria.

Three-Dimensional Printing Enabled Droplet Microfluidic Device for Real-Time Monitoring of Single-Cell Viability and Blebbing Activity.

Droplet-based microfluidics with the characteristics of high throughput, low sample consumption, increasing reaction speed, and homogeneous volume control have been demonstrated as a useful platform for biomedical research and applications. The traditional fabrication methods of droplet microfluidics largely rely on expensive instruments, sophisticated operations, and even the requirement of an ultraclean room. In this manuscript, we present a 3D printing-based droplet microfluidic system with a specifically designed microstructure for droplet generation aimed at developing a more accessible and cost-effective method.

pPe p inhibits HGC-27 cell proliferation, migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway.

Gastric cancer is the fifth most frequently occurring and the fourth most lethal malignant cancer worldwide. A bioactive protein (pPe p) from exhibits significant inhibitory effects on gastric cancer cells. miRNA deep sequencing analysis shows that miR-30b-5p is significantly upregulated in HGC-27 cells treated with pPe p.

A VR-Based Motor Imagery Training System With EMG-Based Real-Time Feedback for Post-Stroke Rehabilitation.

Rehabilitation is essential for post-stroke body function recovery. Supported by the mirror neuron theory, motor imagery (MI) has been proposed as a potential stroke therapy capable of facilitating the rehabilitation. However, it is often quite difficult to estimate the degree of the participation of patients during traditional MI training as well as difficult to evaluate the efficacy of MI based rehabilitation methods.

Regulation of miR-30b in cancer development, apoptosis, and drug resistance.

miR-30b, which is encoded by the gene located on chromosome 8q24.22, plays an important role in a variety of diseases. In most types of tumors, miR-30b significantly inhibits the proliferation, migration, and invasion of cancer cells through the regulation of target genes.

An Immersive Motor Imagery Training System for Post-Stroke Rehabilitation Combining VR and EMG-based Real-Time Feedback.

Motor imagery combining virtual reality (VR) technique has recently been reported to have an increasingly positive impact on post-stroke rehabilitation. However, there is a common problem that the engagement of patients cannot be confirmed during motor imagery training due to a lack of effective feedback control. This paper proposes a VR-based motor imagery training system for post-stroke rehabilitation, using surface electromyographic (EMG)-based real-time feedback to enable the personalized training and quantitative assessment of participation degree.

Anti-Gastric Cancer Effect of Purified Protein via Regulating the JAK/STAT3 Signaling Pathway.

Gastric cancer is the leading cause of cancer-related death worldwide. The aim of present study was to investigate the anti-tumor effect of purified protein (pPep) in gastric cancer. Microarray analysis was performed to find out differentially expressed genes in pPep-treated MC-4 gastric cancer cells.

Gynura divaricata exerts hypoglycemic effects by regulating the PI3K/AKT signaling pathway and fatty acid metabolism signaling pathway.

Objectives: The study aimed to examine the anti-diabetic effects of Gynura divaricata (GD) and the underlying mechanism.

Methods: Information about the chemical compositions of GD was obtained from extensive literature reports. Potential target genes were predicted using PharmMapper and analyzed using Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO).

Rickettsia japonica Infections in Humans, Zhejiang Province, China, 2015.

We investigated 16 Japanese spotted fever cases that occurred in southeastern China during September-October 2015. Patients had fever, rash, eschar, and lymphadenopathy. We confirmed 9 diagnoses and obtained 2 isolates with high identity to Rickettsia japonica strain YH.

Label-free light-sheet microfluidic cytometry for the automatic identification of senescent cells.

Label-free microfluidic cytometry is of increasing interest for single cell analysis due to its advantages of high-throughput, miniaturization, as well as noninvasive detection. Here we develop a next generation label-free light-sheet microfluidic cytometer for single cell analysis by two-dimensional (2D) light scattering measurements. Our cytometer integrates light sheet illumination with a disposable hydrodynamic focusing unit, which can achieve 3D hydrodynamic focusing of a sample fluid to a diameter of 19 micrometer without microfabrication.

Light-sheet-based 2D light scattering cytometry for label-free characterization of senescent cells.

A light-sheet-based 2D light scattering cytometer is developed for label-free characterization of senescent cells. The light-sheet provides an illumination beam with controlled thickness for single cell excitation, and 2D light scattering patterns are obtained by using a defocused imaging method. The principle of this cytometer is validated by distinguishing microspheres with submicron resolution.

Antitumor activity of bacterial exopolysaccharides from the endophyte isolated from .

The endophytic bacterium, MD-b1, was isolated from the medicinal plant and identified as the sp. with 99% similarity based on the partial sequence analysis of 16S rDNA. Exopolysaccharides were extracted from the endophyte for the evaluation of its antitumor activity against gastric carcinoma cell lines (MC-4 and SGC-7901).

A PVP-extract fungal protein of Omphalia lapideacens and its antitumor activity on human gastric tumors and normal cells.

Omphalia lapidescens is an important medicinal fungus as well as traditional Chinese medicine used for disease treatment. It is mainly used as a vermifuge for anthelmintic therapy, but it has not been hitherto reported to possess antitumor activity. In this study, a purified bioactive protein in O.

[Effect of proteins extracted from mycelia of Omphalia lapidescens on inhibiting H, liver cancer in mice and regulating immune function].

Objective: To explore the effect of proteins extraceed from mycelia of Omphalia lapidescens on inhibiting H22 liver cancer in vivo.

Methods: 50 hepatoma 22 tumor bearing mice models were divided into five groups randomly:control group( CG), cyclophosphamide group, and 3 groups of incremental Hepatoma 22 dosages (5, 3, 1 mg/kg). All groups were i.

[Study on endophyte and the growth stages of Ophiopogon japonicus].

Objective: To study on the relationship between the endophyte and the life cycle of Ophiopogon japonicus in Zhejiang.

Methods: Sample roots of Ophiopogon japonicus at different growth stages were thoroughly washed and cut into small fragments, then cleared (removing cytoplasmic contents from cells) using hot 10% KOH and stained with acid fuchsin (alternative stain). The hyphae, the arbuscular and the vesicular of endophyte were examined.

[Isolation and fermentation culture of fungi from Cordyceps soofifera].

Objective: To study the fungi isolated from Cordyceps sobolifera and its fermentation culture.

Methods: The fungi was isolated and identified by its hypha and spores. Three liquid media were used in the culture.

[Study on tissue culture technique of Liriope spicata].

Objective: To study the tissue culture technique of Liriope spicata.

Methods: The tender stem segments with roots as explants were cultured on various media with different kinds of hormones, a lot of bud clusters were induced and grew into seedlings.

Results: A great quantity of whole aseptic seedlings can be formed quickly by the tender stem segments on the media.