Optimized gene-enhanced ERA improves in vitro fertilization outcomes in patients with repeated implantation failure: A meta-analysis.

Background: Assisted reproductive technology still presents a significant challenge when it comes to repeated implantation failure (RIF). To address this issue, personalized embryo transfer (pET) has been suggested as a potential solution. pET involves guiding the embryo transfer process based on an analysis of endometrial receptivity, known as endometrial receptivity analysis (ERA).

"Integrated Stacked" Design "Nanobullet" for High Photothermal Conversion in Dual-Mode Lateral Flow Immunoassay.

(), a prevalent foodborne bacterium, necessitates creating sensitive and rapid detection methods for food safety, with lateral flow immunoassays (LFIAs) using nanomaterials as signal tracers being particularly effective. Enhancements in performance and sensitivity are not restricted to the material alone, we propose an "integrated stacked" concept, which combines amorphous active sites, hollow morphology for enhanced reflection, and symmetric structure for strong absorption resonance. This approach leads to significant photothermal enhancement (η = 60.

Photothermal functionalized antibacterial packaging film with controllable release capability for fruit preservation.

The traditional contact release active packaging film is easily restricted by the strict storage environment and designing a more intelligent release system to achieve lasting food preservation is still a challenge. Herein, a light-responsive thermal-controlled curcumin release packaging film was fabricated by integrating chitosan with Cu-MoO and curcumin (CS/CMC). The addition of filler did not destroy the crystal structure and thermal stability of the chitosan matrix.

Association between inflammatory score, healthy lifestyle, and cardiovascular disease: a national cohort study.

Background: The inflammation score is currently regarded as a reliable composite index for comprehensive assessment of inflammatory status. However, the relationship between inflammation score and cardiovascular disease (CVD) is unclear. Thus, we aimed to explore the association of inflammatory score with CVD, as well as to evaluate whether adhering to a healthy lifestyle could alleviate this association.

Dynamics of hippocampal reactivation for temporal association memory in mice.

Reactivation refers to the re-emergence of activity in neuronal ensembles that were active during information encoding. Hippocampal CA1 neuronal ensembles generate firing activities that encode the temporal association among time-separated events. However, whether and how temporal association memory-related CA1 neuronal ensembles reactivate during sleep and their role in temporal association memory consolidation remain unclear.

Development of attenuated Orf virus as a safe oncolytic viral vector for nasopharyngeal carcinoma treatment.

Background: Orf virus (ORFV) is gaining attention as a promising viral vector for cancer therapy because of its unique properties. Recent studies have shown that ORFV could be effective against various cancers, particularly nasopharyngeal carcinoma. This research explores the ability of wild-type ORFV and recombinant ORFVs, which lack specific virulence factors, to kill NPC cells and modulate the immune response.

Bidirectional Drive Reverse-Phase Enhanced Fluorescence Lateral Flow Immunoassay with Spectral Overlap and Quantitative Balance for the Analysis of Deoxynivalenol.

The capacity of the reverse-phase enhanced fluorescence (turn-on mode) to sensitively alter fluorescence intensity from "zero" to "one" has drawn increasing interest and investigation in competitive lateral flow immunoassay (LFIA). During this process, three important considerations must be made: designing an effective quenching agent, choosing a suitable fluorescence donor, and building a suitable quenching mode. In this work, we employed Au@MnO nanoparticles as highly effective quenchers and AuNCs as fluorescence donors to achieve the overlap of absorption and emission spectra.

Clinical and molecular analysis of JCPyV and BKPyV infection and associated risk of urothelial carcinoma development in the upper tract.

Background: Human polyomaviruses (HPyVs), JC polyomavirus (JCPyV) and BK polyomavirus (BKPyV), have been found in upper tract urothelial carcinoma UTUC; however, the association of the viral oncogenic factors and clinical characteristics of UTUC remains unclear. This study aimed to investigate the prevalence of JCPyV and BKPyV in UTUC and their correlation with cancer progression among the southwest Taiwanese population from 2020 to 2022.

Methods: A total of 72 paraffin-embedded UTUC tissue samples and 41 adjacent tissue samples were collected from 72 patients.

An oral tricyclic STING agonist suppresses tumor growth through remodeling of the immune microenvironment.

Stimulator of interferon genes (STING) agonists could overcome immunosuppressive microenvironment to improve cancer immunotherapy. However, it is challenging to develop oral STING agonists to achieve systemic immunity. In this study, we discovered ZSA-51 as a potent oral STING agonist with distinct benzo[4,5]thieno[2,3-c]pyrrole-1,3-dione scaffold through tricyclic scaffold screening.

Linking the function of -acting RNA elements to coronavirus replication using interactomes.

RNA structures that are functionally important are defined as -acting RNA elements because their functions cannot be compensated for in trans. The -acting RNA elements in the 3' UTR of coronaviruses are important for replication; however, the mechanism linking the -acting RNA elements to their replication function remains to be established. In the present study, a comparison of the biological processes of the interactome and the replication efficiency between the 3' UTR -acting RNA elements in coronaviruses, including severe acute respiratory syndrome coronavirus 2, suggests that (i) the biological processes, including translation, protein folding and protein stabilization, derived from the analysis of the -acting RNA element interactome and (ii) the architecture of the -acting RNA elements and their interactomes are highly correlated with coronavirus replication.

Visible light-driven multichannel concerted antibacterial films with Photodynamically enhanced self-cascading Chemodynamic actions for long-term preservation of perishable food.

This work pioneered an innovative visible light-powered, self-cascading peroxide antimicrobial packaging system (RPFe-CS), featuring a photodynamic enhancement effect achieved through the demand-oriented design of riboflavin sodium phosphate and Fe coordination complexes (RPFe) fillers with photodynamic and peroxidase activities, and the ingenious selection of slightly acidic chitosan (CS) film matrix. In this system, the photo-responsive properties of RPFe particles not only generate the •O, •OH, and O required for photodynamic sterilization, but also the produced HO serves as a necessary substrate for peroxidase to exert its bactericidal effect, endowing the packaging system with a "self-production and self-marketing" cascade process. The RPFe-CS film achieved efficient eradication to bacteria and fungi reaching up to 99.

Discovery of SMD-3236: A Potent, Highly Selective and Efficacious SMARCA2 Degrader for the Treatment of SMARC4-Deficient Human Cancers.

SMARCA2 is an attractive synthetic lethal target in human cancers with mutated, inactivated SMARCA4. We report herein the discovery of highly potent and selective SMARCA2 PROTAC degraders, as exemplified by SMD-3236, which was designed using a new, high-affinity SMARCA ligand and a potent VHL-1 ligand. SMD-3236 achieves DC < 1 nM and > 95% against SMARCA2 and >2000-fold degradation selectivity over SMARCA4.

Transcriptome-wide association identifies as a regulator of mitophagy in non-syndromic cleft lip with or without palate.

This study investigated pathogenic genes associated with non-syndromic cleft lip with or without cleft palate (NSCL/P) through transcriptome-wide association studies (TWAS). By integrating expression quantitative trait loci (eQTL) data with genome-wide association study (GWAS) data, we identified key susceptibility genes, including . Notably, the variant rs12884809 G>A was associated with an increased risk of NSCL/P by enhancing the binding of the transcription factor ELK1 to the promoter, thereby activating its expression.

Manipulating Near-Infrared Absorption via Engineering Anisotropic Plasmonic Spiky Au Nanocubes for the Highly Efficient Dual-Response Immune Detection of T-2 Toxin.

Integrating specific immune recognition, a desirable extinction coefficient, and conspicuous photothermal conversion ability into a single-immune probe to enhance the analysis performance represents an appealing yet significantly challenging task. Herein, by delicately manipulating the geometry of plasmonic nanoparticles from spherical to spiky, precise engineering approach-based spiky Au nanocubes (S-AuNCs) are employed to address this challenge, which fully exploits the plasmon resonance absorption-induced photothermal effect. The finite difference time domain (FDTD) method was employed to computationally simulate the electromagnetic and thermal fields while assessing the feasibility of regulating plasmon resonance for enhanced photothermal absorption.

Discovery of ERD-12310A as an Exceptionally Potent and Orally Efficacious PROTAC Degrader of Estrogen Receptor α (ERα).

Inhibition of estrogen receptor alpha (ERα) signaling is an established therapeutic approach for the treatment of ER-positive (ER+) breast cancers, but new therapeutic strategies are urgently needed to overcome clinical resistance. In the present study, we describe the discovery and extensive evaluation of ERD-12310A as an exceptionally potent and orally efficacious PROTAC degrader of ERα. ERD-12310A achieved a DC value of 47 pM and is 10 times more potent than ARV-471.

Polysaccharides from Cordyceps cicadae Ameliorate Reproductive Impairments in Male Mouse through the Hypothalamic-Pituitary-Testicular Axis.

Scope: Cordyceps cicadae polysaccharides have received attention due to their potential in treating hyperglycemia and enhancing renal function. The beneficial effect of the purified C. cicadae polysaccharides fraction (CCP-1) on the reproductive impairments and spermatogenesis dysfunction of immunocompromised mice is unavailable and is studied herein.

Prophylactic use of probiotics as an adjunctive treatment for ischemic stroke via the gut-spleen-brain axis.

A growing body of research has focused on the role of spleen in orchestrating brain injury through the peripheral immune system following stroke, highlighting the brain-spleen axis as a potential target for mitigating neuronal damage during stroke. The gut microbiota plays a pivotal role in the bidirectional communication between the gut and the brain. Several studies have suggested that probiotic supplements hold promise as a strategic approach to maintaining a balanced intestinal microecology, reducing the apoptosis of intestinal epithelial cells, protecting the intestinal mucosal and blood-brain barrier (BBB), enhancing both intestinal and systemic immune functions, and thereby potentially affecting the pathogenesis and progression of ischemic stroke.

Interactions between polycyclic aromatic hydrocarbons and genetic variants in the cGAS-STING pathway affect the risk of colorectal cancer.

The cGAS-STING pathway plays an essential role in the activation of tumor immune cells. Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants with potential carcinogenicity, and their exposure is associated with the development of colorectal cancer. However, the impacts of genetic factors in the cGAS‒STING pathway and gene‒environment interactions on colorectal cancer remain understudied.

The Novel Application of EUK-134 in Retinal Degeneration: Preventing Mitochondrial Oxidative Stress-Triggered Retinal Pigment Epithelial Cell Apoptosis by Suppressing MAPK/p53 Signaling Pathway.

Age-related macular degeneration (AMD), a leading cause of blindness, is characterized by mitochondrial dysfunction of retinal pigment epithelium (RPE) cells. EUK-134 is a mimetic of SOD2 and catalase, widely used for its antioxidant properties in models of light-induced damage or oxidative stress. However, its effects on the retina are not yet clear.

Jellyfish-Inspired Artificial Spider Silk for Luminous Surgical Sutures.

The development of functional surgical sutures with excellent mechanical properties, good fluorescence, and high cytocompatibility is highly required in the field of medical surgeries. Achieving fibers that simultaneously exhibit high mechanical robustness, good spinnability, and durable fluorescence emission has remained challenging up to now. Taking inspiration from the spinning process of spider silk and the luminescence mechanism of jellyfish, this work reports a luminous artificial spider silk prepared with the aim of balancing the fiber spinnability and mechanical robustness.