[Detection of Angiostrongylus cantonensis larvae with a high resolution melt PCR assay].

Objective: To develop a detection method of the third-stage larvae of Angiostrongylus cantonensis by real-time PCR and high-resolution melt curve analysis.

Methods: A pair of specific primers was designed based on the internal transcribed spacer 1 (ITS1) region of the nuclear ribosomal DNA of A. cantonensis.