Active Components of Pueraria lobata through the MAPK/ERK Signaling Pathway Alleviate Iron Overload in Alcoholic Liver Disease.

Objective: To delve into the primary active ingredients and mechanism of Pueraria lobata for alleviating iron overload in alcoholic liver disease.

Methods: Pueraria lobata's potential targets and signaling pathways in treating alcohol-induced iron overloads were predicted using network pharmacology analysis. Then, animal experiments were used to validate the predictions of network pharmacology.

Integrating network pharmacology and experimental verification to explore the mechanism of puerarin against oliguria in acute alcoholism.

This study was designed to evaluate the pharmacological mechanisms of puerarin against oliguria in acute alcoholism network pharmacology analysis combined with experimental verification. First, this study established an acute alcoholism rat model, compared the changes in urine volume in each group, and observed the therapeutic effect of puerarin by H&E staining, biochemical, RT-qPCR, and immunohistochemical analyses. Second, puerarin-related targets were searched in TCMS, PubChem, CNKI, Wanfang, PubMed, and GeenMedical Academic databases.

Detection of Gegen Adulteration Using Multiple Fingerprints Coupled With Chemometric Strategy.

Background: The root of Pueraria montana var. lobata (gegen) is a well-known traditional Chinese medical herb, which is prone to be accidentally contaminated with Pueraria montana var. thomsonii, Pueraria wallichii, and Pueraria peduncularis due to the morphological character similarity.