[Effects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca(2+) levels in common carp (Cyprinus carpio) pituitary cells in vitro].

In order to study signal transduction in PACAP-stimulated GH release, effects of ovine PACAPs (oPACAPs) on cAMP synthesis and intracellular free Ca(2+)([Ca(2+)](i)) levels in primarily cultured common carp (Cyprinus carpio) pituitary cells were investigated using radioimmunoassay (RIA) for cAMP and [Ca(2+)](i) Indo-1/AM loading techniques. The main results obtained could be briefly summarized as follows: parallel with their GH-releasing effects, oPACAP-38 (0.01 nmol/L to 1 micromol/L) and oPACAP-27 (0.