Highly sensitive time-resolved fluorescent microspheres lateral flow immunoassay for the quantitative detection of triadimefon and its metabolite residues in fruits and vegetables.

A general one-step lateral flow immunochromatographic assay (LFIA) for the quantitative detection of triadimefon (TDF) and triadimenol (TDN) in fruit and vegetable samples was developed using time-resolved fluorescence microspheres (TRFM) as labels. A specific anti-triadimefon monoclonal antibody (mAb) was conjugated with TRFM to fabricate LFIA test strips. A time-resolved fluorometer as an LFIA reader was applied to obtain quantitative results and assess risk ranges for the LFIA test strips.

[Distribution of Press-sensitive Acupoints in Patients with Tinnitus].

Objective: To explore the regularity of distribution of press-sensitive acupoints on the body surface in tinnitus patients, so as to provide evidence for selecting suitable acupuncture points in the treatment of tinnitus.

Methods: Sixty-three cases of tinnitus patients (from 25 to 75 years in age) were recruited in the present study. The patient was asked to take a sitting, supine and prone position, successively, and the operator used the thumb to moderately press the subject's head, neck, back, four limbs below the knee- and elbow joints along the three Hand- and three Foot- and meridians.