[Detection and quantification of aberrant leukemia fusion gene by real-time RT-PCR].

This study was purposed to set up real-time quantitative RT-PCR technique and to measure leukemia fusion gene transcripts in patients with chronic myeloid leukemia (CML), acute lymphoblastic leukemia (ALL) and acute promyelocytic leukemia (APL). All plasmids containing the target gene sequences were constructed to establish the standard curves. A TaqMan based real-time quantitative RT-PCR was performed to measure aberrant fusion gene transcripts in 130 samples of peripheral blood (PB) or bone marrow (BM) from 49 patients with leukemia.

[Apoptosis and cell cycle arrest in lymphoma Raji cells induced by arsenic trioxide].

This study was aimed to investigate the apoptosis induced by arsenic trioxide (As2O3) in lymphoma Raji cells and its possible mechanisms. The inhibitory effect of different concentration of As2O3 on cell proliferation was tested by MTT assay. Apoptosis was observed with electron microscope and DNA electrophoresis.

[Inhibited proliferation of B-lymphoma Raji cells and down-regulated expression of VEGF by arsenic trioxide].

This study was aimed to investigate the effects of As2O3 on proliferation of B lymphoma Raji cell and to study the expression changes of VEGF mRNA. The modified MTT was adopted to evaluate the effect of As2O3 on proliferation of Raji cells. Semi-quantitative RT-PCR was used to detect the expression of VEGF121 and VEGF165 mRNA in Raji cells exposed to As2O3.