Determination of tetrodotoxin in bivalve mollusks by hydrophilic interaction liquid chromatography-triple quadrupole mass spectrometry with internal standard calibration and its contamination in Zhejiang province, China.

A method was established for determining tetrodotoxin (TTX) in bivalve mollusks by hydrophilic interaction liquid chromatography-triple quadrupole mass spectrometry (HILIC-MS/MS) using kasugamycin as the internal standard for quantification. Samples were subjected to ultrasonic extraction with methanol-water (1:1, v/v) containing 0.5% acetic acid, protein precipitation with acetonitrile, clean-up using a cation exchange solid phase extraction cartridge, elution with acetonitrile:water (1:1, v/v) containing 0.

[Occurrence and Spatial Distribution of Volatile Organic Compounds in Urban Drinking Water Distribution Systems].

Volatile organic compounds (VOCs) in the urban drinking water distribution systems for two coastal cities (S City and L City) in China were analyzed. Health risk assessments of VOCs detected in tap water were performed, and the distribution characteristics of VOCs in the tap water pipe network were also investigated. Among 47 target VOCs, 16 compounds were detected, of which 11 were detected in both S City and L City.

Modeling the transport of TiO2 nanoparticle aggregates in saturated and unsaturated granular media: effects of ionic strength and pH.

This study aims to explore the mechanisms governing the transport and retention kinetics of TiO(2) nanoparticle aggregates (NPAs) in flow-through columns of packed sand, particularly under unsaturated conditions. The study was carried out at different pHs (2.6, 7.

[Effect of low-dose hydroxyurea with sodium butyrate on globin gene expression in human erythroid progenitor cells].

Objective: To investigate the effects of combined use of low-dose hydroxyurea (HU) and sodium butyrate (NaB) on the expression of 7 globin genes (zeta, alpha, epsilon, Ggamma, Agamma, delta, and beta) in human erythroid progenitor cells.

Methods: Human erythroid progenitor cells were cultured using a two-step liquid culture system and treated with HU and NaB either alone or in combination. The inhibitory effects of the agents on the cell growth were monitored with trypan blue exclusion assay, and the changes in the mRNA of the 7 globin genes were detected using RT-PCR.

[Mapping QTLs related to head length, eye diameter and eye cross of common carp (Cyprinus carpio L.)].

A common carp gynogenetic line including 44 individuals derived from the cross Barbless carp x Hebao-cold tolerance red carp was used to construct a linkage map using 445 markers (265 AFLP markers, 127 SSR markers, 37 EST-SSR markers and 16 RAPD markers). Quantitative traits loci (QTLs) associated with head length, eye diameter, and eye cross were identified by composite interval mapping of the software WinQTLCart2.5.