Detection of auto antibodies and transplantation of cultured autologous melanocytes for the treatment of vitiligo.

The aim of the present study was to establish an immunofluorescence method of antibody detection to identify melanocytes in the serum of vitiligo patients. Furthermore, we aimed to establish a method for the culture and proliferation of autologous pure melanocytes and to observe the effect of their transplantation for the treatment of vitiligo. Suspension of epidermal cells with melanocytes was performed using trypsin digestion of normal epiderm from eyelid operation and melanocytes were selectively cultured and proliferated in serum-free M2 medium.

Hydroxysafflor yellow A enhances survival of vascular endothelial cells under hypoxia via upregulation of the HIF-1 alpha-VEGF pathway and regulation of Bcl-2/Bax.

Hydroxysafflor yellow A (HSYA) is a component of the flower Carthamus tinctorius L. The present investigation determines whether HSYA can modify the effects of hypoxia on vascular endothelial cells (EC) and its mechanisms. Human EC line (EAhy926) viability was determined using the MTT assay.

[Expression and activity of tissue-type plasminogen activator mutant reteplase with deletion of PAI-1 binding sites].

Aim: To construct a mutant of human tissue plasminogen activator (t-PA) with activity not inhibited by plasminogen activator inhibitor-1 (PAI-1).

Methods: The finger, epidermal growth factor and kringle-1 domains were removed from native t-PA to obtain a t-PA mutant with the PAI-1 binding sites. A pUC18 plasmid containing the human t-PA full-length cDNA sequence was used as template, and the DNA sequences encoding 1-3 and 176-527 amino acids were amplified by PCR.

[Prokaryotic expression of DnaJ-homologous chaperon PBP and preparation of rabbit antibody against PBP].

Aim: To express DnaJ-homologous chaperon peripherin-binding protein(PBP) gene in E.coli and prepare the rabbit antibody against PBP.

Methods: The PBP cDNA was amplified from the human fetal brain tissue by RT-PCR.

[Cloning of a DnaJ homolog chaperon PBP and its subcellular localization].

Aim: To isolate and identify a human DnaJ homolog chaperon, PBP, from a human skeleton cDNA library, and to analyze its expression and distribution in transfected mammalian cells.

Methods: (32)p-dCTP labeled probe hybridization was used to screen the human skeleton cDNA library and sequence of the positive clones were analyzed. Then PBP gene was transfected into COS-7 cells using lipofectamin.

[Construction of a cDNA subtractive library of rat brain after repeated +Gz exposure].

Objective. To construct a cDNA subtractive library of rat brain after repeated + Gz exposures with suppression subtractive hybridization (SSH). Method.