Exploring the potential of solid and liquid amniotic membrane biomaterial in 3D models for prostate cancer research: A comparative analysis with 2D models.

Objective: Research and tools are necessary for understanding prostate cancer biology. 3D cell culture models have been created to overcome the limitations of animal models and 2D cell culture. The amniotic membrane (AM), a natural biomaterial, emerges as an ideal scaffold for 3D cultures due to its accessibility and incorporation of the extracellular matrix (ECM) in both solid and liquid forms.