Application of an Anchor Mapping of Alien Chromosome (AMAC) Fragment Localization Method in the Identification of Radish Chromosome Segments in the Progeny of Rape-Radish Interspecific Hybrids.

Rape () is an important oilseed crop widely cultivated worldwide. Due to its relatively short evolutionary and domestication history, its intra-species genetic diversity is limited. Radish (), belonging to a different genus but the same family as , possesses an abundance of excellent gene resources.

Molecular diversity and phylogenetic analysis of ten sheep breeds from Khyber Pakhtunkhwa, Pakistan, based on mitochondrial D-loop sequences.

Livestock farming has a key role in many rural communities both economically and culturally. It plays an important role in overcoming the deficiencies of meat, milk, wool and various by-products. Pakistan has a large number of livestock, well-adapted to local conditions.

A study on the performance of enhancing environmental protection from molecular iodine using granular impregnated activated carbon.

Environmentally hazardous radioactive isotopes of iodine may be released from a nuclear power plant as a by-product of uranium fission. The efficient and safe capture of volatile radioiodine is of great significance in the history of nuclear power plants. Due to its high volatility and carcinogenic characteristics, elimination of iodine gas (I) from air is the need of the hour from an environmental and health point of view.

COMPARISON OF EFFECTIVENESS OF INFERIOR ALVEOLAR NERVE BLOCK AND GOW GATES TECHNIQUE IN MANDIBULAR MOLAR TEETH WITH IRREVERSIBLE PULPITIS.

Background: Local anaesthetics are the most important and most utilized agents in the dental profession today. Achieving effective pain control holds a prime importance for any dental procedure. The most commonly employed technique to achieve effective anaesthesia in mandibular molar teeth with irreversible pulpitis is Inferior Alveolar Nerve Block.

Machining performance, economic and environmental analyses and multi-criteria optimization of electric discharge machining for SS310 alloy.

With the expansion of the manufacturing sector, it has become crucial to incorporate sustainable production methods in order to remain competitive in the market. This study focuses on addressing the needs of the manufacturing industry by conducting a sustainability analysis of electric discharge machining for SS310 alloy. The analysis explores the impact of various electrode materials, for instance, copper and brass, as well as different machining variables, including discharge current (I = 4-12 A), spark gap (SG = 6-12 mu), pulse duration (Pon = 15-45 μs), and duty cycle (DC = 75-85%) using Taguchi method.

Teacher vaccinations enhance student achievement in Pakistan: The role of role models and theory of mind.

We provide experimental evidence that role models can galvanize prosocial actions amid global crises, exemplified by the COVID-19 pandemic. In a randomized control trial comparing role models, cash incentives, and celebrity endorsements, only role models successfully mitigated vaccine reluctance and ameliorated pandemic-induced educational setbacks. Monthly tracking of vaccination status was achieved via QR-code-verified certificates.

Assessment of submicron bone tissue composition in plastic-embedded samples using optical photothermal infrared (O-PTIR) spectral imaging and machine learning.

Understanding the composition of bone tissue at the submicron level is crucial to elucidate factors contributing to bone disease and fragility. Here, we introduce a novel approach utilizing optical photothermal infrared (O-PTIR) spectroscopy and imaging coupled with machine learning analysis to assess bone tissue composition at 500 nm spatial resolution. This approach was used to evaluate thick bone samples embedded in typical poly(methyl methacrylate) (PMMA) blocks, eliminating the need for cumbersome thin sectioning.

How Cultural Involvement in Different Brand Types Influences Willingness to Pay Premium: The Mediating Role of Brand Happiness.

This study examines the impact of cultural involvement on brand enriching benefits and consumers' willingness to pay a premium (WPP). Additionally, it explores the underlying mechanisms behind this impact. A partial least squares structural equation model was used to analyze the data collected from a pretest (N = 70) and an online survey (N = 1053) conducted in China.

Impact of dietary exposure to polyester microfibers on hematology, serology and histology in a mouse model.

Synthetic fabrics, especially polyester, are a primary source of microplastic fibers (MFs), but there is limited data on their accumulation and dose related health impact in living organisms. This study examined the effects of ingested polyester microfibers (PE-MFs) on hematology, histopathology, and serum biochemistry in albino mice. Mice were given varying doses of PE-MFs (100, 200, 400 and 800 μg/d/mice) for a duration of thirty-five days and a notable decreases in certain hematological parameters such as RBCs, Hb, and platelets, and increases in MCV and MCH was noted at (p < 0.

Predictors of Ischemic Stroke After Acute Coronary Syndrome: A Retrospective Analysis.

Background Ischemic stroke (IS) is a fatal complication of acute coronary syndrome (ACS). Factors that speed up IS development after ACS are understudied, especially in developing countries like Pakistan. Thus, this study was designed to identify the potential risk factors of IS in patients with a preceding episode of ACS.

Integrative approaches to improve litchi (Litchi chinensis Sonn.) plant health using bio-transformations and entomopathogenic fungi.

Bio-transformations refer to the chemical modifications made by an organism on a chemical compound that often involves the interaction of plants with microbes to alter the chemical composition of soil or plant. Integrating bio-transformations and entomopathogenic fungi into litchi cultivation can enhance symbiotic relationships, microbial enzymatic activity in rhizosphere, disease suppression and promote overall plant health. The integration of biological formulations and entomopathogenic fungi can significantly influence growth, nutrient dynamics, physiology, and rhizosphere microbiome of air-layered litchi (Litchi chinensis Sonn.

Effects of Different Photoperiods during Incubation on Post-Hatch Broiler Performance and Stress Response.

This study evaluated the subsequent effect of photoperiods during incubation on post-hatch growth and stress response of commercial broiler chickens. A total of 875 Ross 308 broiler breeder (48 weeks of age) eggs were hatched using different durations (0, 4, 8, 12, 16, 20, and 24 h a day) of dichromatic light [green and red (495 to 750 nm); 2700 K; 250 lux; SUNJIE; China] throughout the whole period of incubation. A total of 50 0-day-old hatched straight run broiler chicks from each photoperiod during incubation were used to evaluate subsequent growth performance (feed intake, body weight, and feed conversion ratio); stress parameters (physical asymmetry, tonic immobility, and vocalization,); welfare traits (feather score and gait score); carcass traits (live weight, dressed weight, carcass yield, liver weight, gizzard weight, heart weight, abdominal fat weight, breast weight, and leg weight); and serum chemistry (globulin, total protein, cholesterol, glucose, and uric acid).

Photovoltaic properties of halide perovskites for solar cell application with efficiency greater than 18.

The opto-electronic properties and solar cell efficiency of halide perovskites ALiInBr (A = Rb, Cs) are investigated using density functional theory (DFT) through WEIN2k and SCAPS-1D. The electronic characteristic of ALiInBr (A = Rb, Cs) compounds reveal their direct bandgap semiconductor nature and are active in visible rang. The results indicate that substituting Cs with Rb causes a slight narrowing of the bandgap.

Crystal structure of Kunitz-type trypsin inhibitor: Entomotoxic effect of native and encapsulated protein targeting gut trypsin of Herbst.

Trypsin inhibitors are known to act against insect pests by inhibiting proteases of the digestive tract. In this study, we report structural and functional characterization of ∼ 19 kDa Kunitz-type trypsin inhibitor (ApKTI) protein with potential bio-insecticidal applications. Crystal structure of ApKTI protein has been refined to 1.

Exploring Acyl Thiotriazinoindole Based Pharmacophores: Design, Synthesis, and SAR Studies with Molecular Docking and Biological Activity Profiling against Urease, α-amylase, α-glucosidase, Antimicrobial, and Antioxidant Targets.

A diminutive chemical library of acyl thiotriazinoindole (ATTI) based bioactive scaffolds was synthesized, instigated by taking the economical starting material Isatin, through a series of five steps. Isatin was first nitrated followed by the attachment of pentyl moiety via nucleophilic substitution reaction. The obtained compound was reacted with thiosemicarbazide to obtain thiosemicarbazone derivative, which was eventually cyclized using basic conditions in water as solvent.

Interleukin-6 (-174G/C), Interleukin-1β (-511 C/T), and Apolipoprotein B-100 (2488 C/T) Gene Polymorphism in Pre-Eclampsia.

: Pre-eclampsia (PE) is a pregnancy-specific condition characterized by significant health risks for pregnant women worldwide due to its status as a multi-organ disorder. High blood pressure (hypertension) with or without proteinuria is usually considered an initial clinical sign of PE. The pathogenesis of pre-eclampsia is highly complex and likely involves multiple factors, including poorly developed uterine spiral arterioles, immunological issues, placental ischemia or infarction, and genetic abnormalities.

Unraveling the molecular landscape of lead-induced cochlear synaptopathy: a quantitative proteomics analysis.

Introduction: Exposure to heavy metal lead can cause serious health effects such as developmental neurotoxicity in infants, cognitive impairment in children, and cardiovascular and nephrotoxic effects in adults. Hearing loss is one of the toxic effects induced by exposure to lead. Previous studies demonstrated that exposure to lead causes oxidative stress in the cochlea and disrupts ribbon synapses in the inner hair cells.

Efficacy of Faculty Development Training Workshops (FDTWs) on Writing High-Quality Multiple-Choice Questions at Northern Border University (NBU) in the Kingdom of Saudi Arabia (KSA).

Background: A multiple-choice question (MCQ) is a frequently used assessment tool in medical education for both certification and competitive examinations. Well-constructed MCQs impact the utility of the assessment and, thus, the fate of the examinee.

Aims And Objectives: To analyze the basic science faculty perceptions of writing high-quality MCQs, to create awareness of item-writing flaws in constructing high-quality MCQs, and to determine the impact of faculty development training workshops (FDTWs) on MCQ writing skills.

KRAS and BRAF genetic alterations in lung cancer: A case - control study.

Context: Lung cancer (LC) is one of the most critical neoplastic abnormalities, having globally a high mortality rate. Knowledge about its genetic mutations and their association with clinically pathological features of LC is very important. Here, we describe the epidemiological molecular study of genetic mutations in KRAS and BRAF genes and their relationship with the demographic and clinical characteristics of Pakistani patients with lung adenocarcinoma.