Evaluation of raw milk samples and vendor-derived and prevalence in dairy delicatessens in eastern Turkey.

This study investigated the prevalence, antimicrobial resistance, and genetic diversity of , as well as the detection and genetic relationship of in retail milk samples and plastic bags from 25 local dairy delicatessens. Bacteriological culture, PCR, and Sanger sequencing were performed. Eleven percent of the samples were positive for , none of which were methicillin-resistant or enterotoxigenic.

A Longitudinal Study on in Conventionally Reared Commercial Broiler Flocks in the United States: Prevalence and Genetic Diversity.

Poultry meat contaminated with , a major bacterial cause of foodborne gastroenteritis worldwide, is considered the primary source of human campylobacteriosis. Thus, reduction or elimination of in poultry production will have a significant impact on food safety and public health. Despite the significant progress made over the last decades, many puzzles remain about the epidemiology of on poultry farms, hampering the development of an effective control strategy.

Prevalence and antimicrobial resistance of Campylobacter species in shelter-housed healthy and diarrheic cats and dogs in Turkey.

Background: Campylobacter spp. are among the leading foodborne bacterial pathogens. Pet animals may be an important reservoir for human infection.

Prevalence and clonal diversity of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats with eye discharge.

Objectives: Methicillin-resistant Staphylococcus (MRS) has originated, spread extensively, and become a prominent source of bacterial infections in both human and animal.

Methods: We report the prevalence, genetic diversity, and antimicrobial resistance pattern of Staphylococcus pseudintermedius and Staphylococcus aureus strains isolated from dogs and cats with eye discharges.

Results: A total of 12 (6.

Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in in Calves.

Fluoroquinolone (FQ) resistance in a major foodborne bacterial pathogen, , derived from cattle has recently become prevalent and poses a significant public health concern. However, the underlying factors for this increase are not entirely clear. To evaluate the effect of enrofloxacin treatment on FQ-resistance development in , 35 commercial calves were equally divided into five groups (Groups 1-5) and were orally inoculated with FQ-susceptible (FQ-S) .

Nanoemulsion-Based Hydrogels and Organogels Containing Propolis and Dexpanthenol: Preparation, Characterization, and Comparative Evaluation of Stability, Antimicrobial, and Cytotoxic Properties.

Recently, nanoemulsion-based gels have become very popular for dermal drug delivery, overcoming the disadvantages of conventional semi-solid drug forms. The aim of this study is to prepare and characterize nanoemulsion-based hydrogels and organogels containing combined propolis and dexpanthenol, and to compare their stability, antimicrobial, and cytotoxicity properties. Within the scope of characterization studies, organoleptic properties, drug content, morphology, pH, gel-sol conversion temperature, spreadability, viscosity, FT-IR, and release properties were evaluated in hydrogels and organogels.

Prevalence, Mechanism, Genetic Diversity, and Cross-Resistance Patterns of Methicillin-Resistant Isolated from Companion Animal Clinical Samples Submitted to a Veterinary Diagnostic Laboratory in the Midwestern United States.

Methicillin-resistant Staphylococcus (MRS) is a leading cause of skin and soft tissue infections in companion animals, with limited treatment options available due to the frequent cross-resistance of MRS to other antibiotics. In this study, we report the prevalence, species distribution, genetic diversity, resistance mechanism and cross-resistance patterns of MRS isolated from companion animal (mostly dog and cat) clinical cases submitted to Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) between 2012 and 2019. The majority of isolates were identified as Staphylococcus pseudintermedius (68.

Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in in Calves.

is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in , 30 commercial calves were divided into Group 1, Group 2, and Group 3 ( = 10), and were all inoculated orally with FQ-susceptible (FQ-S) ; seven days later, Group 3 was challenged with transtracheal , and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin.

Distribution of CRISPR Types in Fluoroquinolone-Resistant Isolates.

To aid development of phage therapy against , we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant . A total of 100 FQ-resistant strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gene and the presence of various CRISPR systems. All but one isolate harbored 1-5 point mutations in and the most common mutation was the Thr86Ile change.

Bacterial Cross-Contamination in a Veterinary Ophthalmology Setting.

The present study describes the prevalence of bacterial cross-contamination in a veterinary ophthalmology setting, a serious issue that can result in healthcare-associated (or nosocomial) infections among patients and staff. Retrospective ( = 5 patients) and prospective ( = 23 patients) studies evaluated bacterial isolates in companion animals presenting with ulcerative keratitis, sampling the patients' cornea and surrounding examination room, including the environment (exam table, countertop, floor) and ophthalmic equipment (slit lamp, transilluminator, direct ophthalmoscope, indirect headset, tonometer). Results of bacterial culture and antibiotic susceptibility testing were recorded, and degree of genetic relatedness was evaluated in six pairs of isolates (cornea + environment or equipment) using pulse-field gel electrophoresis (PFGE).

Prevalence of Colistin Resistance in in Eastern Turkey and Genomic Characterization of an Positive Strain from Retail Chicken Meat.

Colistin is one of the most effective antibiotics against multidrug resistant Gram-negative bacteria. However, the recent emergence of plasmid-borne mobilized colistin resistance () genes is considered a serious antimicrobial resistance challenge worldwide. In this study, we report detection of an carrying isolate (named ATAVET -1 Turkey) from retail raw chicken meat in Turkey.

Phenotypic and Genotypic Examination of Antimicrobial Resistance in Thermophilic Species Isolated from Poultry in Turkey.

Introduction: The study aimed to isolate thermophilic from chickens raised three rearing methods, determine its antimicrobial susceptibilities, and examine resistance-related genes by PCR.

Material And Methods: Cloacal swabs or intestinal contents were taken in Istanbul, Sakarya, and Izmir provinces. Chickens were from small village-based family-run businesses (n = 70), organically raised (n = 71), and conventionally raised broilers (n = 79).