Chemometric modeling of the electrochemical data to investigate proline cis/trans isomeration effect on aggregation of Tau protein.

Tau protein (Tau) is a proline-rich protein and in this work, we have developed a very interesting strategy based on combination of electrochemistry with chemometric methods to investigate proline cis/trans isomeration effect on the Tau aggregation. To achieve this goal, the proline residues at RTPPK motif have been replaced by alanine to generate RTPAK, RTAPK and RTAAK mutants of the Tau. Then, cyclic voltammetric (CV) responses of the Tau and RTPAK, RTAPK and RTAAK as its mutants in the presence of heparin (HEP) as an anionic inducing agent which could trigger aggregation of the Tau were recorded at physiological conditions every hour during 12 h.