Microbial community response to biostimulation and bioaugmentation in crude oil-polluted sediments of the Persian Gulf.

The Persian Gulf is a transit point for a lot of crude oil at the international level. The purpose of this research is to compare two methods of biostimulation and bioaugmentation for degradation of sediments contaminated with crude oil in the Persian Gulf. In this research, six types of microcosms were designed (Sediments from Khark Island).

Isolation and Identification of Phenol-Degrading Bacteria from Iranian Soil and Leaf Samples.

Background: By considering the importance and role of soil in the health of humanity, it is important to remove the presence of harmful compounds, such as phenol.

Methods: In this study, four types of soil and leaf samples were collected from Kerman, Iran, and the amounts of heterotrophic and degradation bacteria were determined using the serial dilution and most probable number (MPN) methods. The amount of removed phenol was investigated using the Gibbs method with different concentrations of phenol.

Glycolipopeptide biosurfactant from SG: physicochemical characterization, optimization, antibiofilm and antimicrobial activity evaluation.

The SG isolated from soil samples at the Persian Gulf was analyzed for its ability to produce biosurfactant. Various screening techniques were used for evaluating biosurfactant production and confirming biosurfactant presence in the culture supernatant. Most n-alkanes in the bacterial culture media were effectively degraded in the presence of biosurfactant acquired from the bacteria.

Investigation of the synergistic effect of glycolipid biosurfactant produced by Shewanella algae with some antibiotics against planktonic and biofilm forms of MRSA and antibiotic resistant Acinetobacter baumannii.

To tackling antibiotic resistance and the appearance of multidrug-resistant (MDR) strains, one current approach is the combined use of biosurfactants with antibiotics to increase their efficacy. The antimicrobial ability of biosurfactant produced by Shewanella algae strain B12 was examined using the agar well diffusion method versus some resistant Gram-negative and Gram-positive bacteria. The Minimum Inhibitory Concentration (MIC) of Glycolipid-Biosurfactant of B12 (GBB12) was performed by the broth dilution technique.

Isolation, Optimization, and Structural Characterization of Glycolipid Biosurfactant Produced by Marine Isolate Shewanella algae B12 and Evaluation of Its Antimicrobial and Anti-biofilm Activity.

Biosurfactants are microbial-derived compounds with surface and emulsifying activities. Environmental and industrial applications make glycolipid biosurfactants particularly interesting among the several categories of biosurfactants. A potential glycolipid biosurfactant resource, Shewanella algae, was isolated from marine samples at the Persian Gulf.

One-pot synthesis and biochemical characterization of a magnetic collagenase nanoflower and evaluation of its biotechnological applications.

Recently, hierarchical magnetic enzyme nanoflowers have been found extensive attention for efficient enzyme immobilization due to high surface area, low mass transfer limitations, active site accessibility, promotion of the enzymatic performance, and facile reusing. Herein, we report the purification of the Bacillus collagenase and then synthesis of magnetic cross-linked collagenase-metal hybrid nanoflowers (mcCNFs). The catalytic efficiency (k/K) value of the immobilized collagenase was 2.

Crude oil pollution and biodegradation at the Persian Gulf: A comprehensive and review study.

The Persian Gulf consider as the fundamental biological marine condition between the seas. There is a different assortment of marine life forms including corals, wipes, and fish in this marine condition. Mangrove timberlands are found all through this sea-going biological system.

Formulation and characterization of nanoemulsion from essential oil and study of its antimicrobial, antibiofilm, and plasmid curing activity against antibiotic-resistant pathogenic bacteria.

Nanoemulsion technology is an alternative candidate to overcome antibiotic resistance in pathogenic bacteria. The aim of this research was nanoemulsion production from the essential oil of and the characterization of this nanostructure. Nanoemulsion of essential oil from was prepared using the ionotropic gelation method and chitosan as a nano-carrier.

Diversity of halophilic and halotolerant bacteria in the largest seasonal hypersaline lake (Aran-Bidgol-Iran).

Purpose: In this study, the culturable halophilic and halotolerant bacterial diversity was determined in Aran-Bidgol as a thalassohaline seasonal hypersaline lake in Iran.

Methods: Thirty water, soil, sediments, coastal mud, multi-color brines and salt crystals samples were extracted and cultured using different media and incubation conditions. Totally 958 isolates were obtained and 87 isolates were selected for further studies, based on morphological, physiological and biochemical tests, representing different morphotypes.

Dual catalytic DNA circuit-induced gold nanoparticle aggregation: An enzyme-free and colorimetric strategy for amplified detection of nucleic acids.

An enzyme-free dual catalytic DNA circuit for amplified detection of nucleic acids has been developed. The system functions based on a cyclic self-assembly of two auxiliary hairpins (H1 and H2) and three biotinylated hairpin oligonucleotides (H3, H4 and H5), in the format of two molecular circuits. In the upstream circuit, a target initiator (I) besides H1 and H2 hairpins constructs H1-H2 duplexes that trigger the operation of a subsequent circuit.

Degradation of phenol at high concentrations using immobilization of Pseudomonas putida P53 into sawdust entrapped in sodium-alginate beads.

Phenols are distributed either as natural or artificial mono-aromatic compounds in various environmental sites as major pollutants. The objective of this study was the immobilization of the phenol degrading bacteria P. putida P53 and A.

Study the antibacterial and antibiofilm activity of Carum copticum against antibiotic-resistant bacteria in planktonic and biofilm forms.

Objectives: Microbial biofilms has attracted interest in the recent years because they has become the most important cause of nosocomial infections. This study was aimed to examine the antibacterial activities of Carum copticum extracts on the development of microbial biofilms and planktonic form of six pathogenic bacteria.

Methods: Antimicrobial activity of the crude extracts against the planktonic form of six pathogenic bacteria: Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii and Klebsiella pneumonia was evaluated by using the disc diffusion method.

Antibacterial Activity of (Essential Oil) Against Pathogenic Bacteria and Determination of Chemical Composition of Essential Oils by Gas Chromatography-Mass Spectrometry Analysis in Four Regions of Kerman.

The use of medicinal plants has been considered due to increasing bacterial resistance to antibiotics. is a plant with high potential in medicine. The aim of this study was to evaluate the antibacterial activities and gas chromatography-mass spectrometry (GC-MS) analyses of the essential oil of from four regions in Kerman: Hezar Mountain, Laleh Zar, Bidkhan, and Rabor.

Production and characterization of an acido-thermophilic, organic solvent stable cellulase from HSC7 by conversion of lignocellulosic wastes.

The acidophilic and thermophilic cellulase would facilitate the conversion of lignocellulosic biomass to biofuel. In this study, HSC7 isolated as the best thermophilic cellulose degrading bacterium from Gorooh hot spring. 16S rRNA gene sequencing showed that, this strain closely related to the .

Purification and biochemical properties of a thermostable, haloalkaline cellulase from AMF-07 and its application for hydrolysis of different cellulosic substrates to bioethanol production.

A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa.

Antibacterial Activity of Euphorbia hebecarpa Alcoholic Extracts Against Six Human Pathogenic Bacteria in Planktonic and Biofilm Forms.

Background: Biofilm formation is a primary cause of considerable bacterial destruction.

Objectives: In an effort to combat these industrial and medical bacterial biofilm problems, our study aims to determine the antimicrobial effect of Euphorbia hebecarpa.

Materials And Methods: The inhibition efficiency of alcoholic extracts on the planktonic form of six pathogenic bacteria was evaluated using a disk diffusion technique.

Comparison the effects of bioaugmentation versus biostimulation on marine microbial community by PCR-DGGE: A mesocosm scale.

In order to better understand the effects of biostimulation and bioaugmentation processes on a marine microbial community, three different mesocosm experiments were planned. Natural seawater (10.000L) was artificially polluted with crude oil (1L) and (1) inorganic nutrients (Biostimulating Mesocosm, BM), (2) inorganic nutrients and an inoculum of Alcanivorax borkumensis SK2 (Single Bioaugmentation Mesocosm, SBM), (3) inorganic nutrients and inoculums of A.

Enrichment and identification of naphthalene-degrading bacteria from the Persian Gulf.

Naphthalene is a ubiquitous pollutant of the marine environment, and naphthalene biodegradation has been receiving constant scientific consideration. For cleanup of aromatic contaminated sites, bioremediation methods are considered as economical and safe approaches for the marine environment. The aims of this research are isolation and characterization of naphthalene-degrading bacteria from some marine samples of the Persian Gulf.

Study the symbiotic crude oil-degrading bacteria in the mussel Mactra stultorum collected from the Persian Gulf.

Symbiotic associations are complex partnerships that can lead to new metabolic capabilities and the establishment of novel organisms. The diversity of these associations is very broad and there are still many mysteries about the origin and the exact relationship between the organisms that are involved in a symbiosis. The aim of the present study is to find symbiotic crude-oil degrading bacteria in the mussels that collected from the Persian Gulf.

Isolation and characterization of phenol degrading yeasts from wastewater in the coking plant of Zarand, Kerman.

Phenol and phenolic compounds are environmental pollutants present in industrial wastewaters such as coal tar, oil refineries and petrochemical plants. Phenol removal from industrial effluents is extremely important for the protection of environment. Usually, phenol degradation is carried out by physicochemical methods that are costly and produce hazardous metabolites.

Immobilization of Microbes for Bioremediation of Crude Oil Polluted Environments: A Mini Review.

Petroleum hydrocarbons are the most common environmental pollutants in the world and oil spills pose a great hazard to terrestrial and marine ecosystems. Oil pollution may arise either accidentally or operationally whenever oil is produced, transported, stored and processed or used at sea or on land. Oil spills are a major menace to the environment as they severely damage the surrounding ecosystems.

Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf.

To date, little is known about existing relationships between mussels and bacteria in hydrocarbon-contaminated marine environments. The aim of this study is to find crude oil degrading bacteria in some mussels at the Persian Gulf. Twenty eight crude oil degrading bacteria were isolated from three mussels species collected from oil contaminated area at Persian Gulf.

The Effects of Allium sativum Extracts on Biofilm Formation and Activities of Six Pathogenic Bacteria.

Background: Garlic is considered a rich source of many compounds, which shows antimicrobial effects. The ability of microorganisms to adhere to both biotic and abiotic surfaces and to form biofilm is responsible for a number of diseases of chronic nature, demonstrating extremely high resistance to antibiotics. Bacterial biofilms are complex communities of sessile microorganisms, embedded in an extracellular matrix and irreversibly attached to various surfaces.

The inhibitory effect of Thymus vulgaris extracts on the planktonic form and biofilm structures of six human pathogenic bacteria.

Objective: Microorganisms are responsible for many problems in industry and medicine because of biofilm formation. Therefore, this study was aimed to examine the effect of Thymus vulgaris (T. vulgaris) extracts on the planktonic form and biofilm structures of six pathogenic bacteria.