A fires novel report of exosomal electrochemical sensor for sensing micro RNAs by using multi covalent attachment p19 with high sensitivity.

Exosomes are natural spherical phospholipids vesicles derived from cells. Dropping MCF-7 exosome (with high biomarkers as exosomal case) covalently onto the SCPE-GNP causes the sensor to be stable due to the electrochemical induction of positive charges of different biomarkers with [Fe(CN)6] reactions. In the following, the covalent p19 connection with the biomarkers of exosome turns off the sensor.

Co-infection of Mycobacterium tuberculosis and Pneumocystis jirovecii in the Iranian Patients With Human Immunodeficiency Virus.

Background: Based on the authors' knowledge, there is no study on the co-infection of opportunistic agents such as Mycobacterium tuberculosis and Pneumocystis jirovecii in the lungs of Iranian patients with immunosuppression.

Objectives: The current study aimed to show the rate of co-infection of M. tuberculosis and P.

Dihydropteroate synthase gene mutation rates in Pneumocystis jirovecii strains obtained from Iranian HIV-positive and non-HIV-positive patients.

The dihydropteroate sulfate (DHPS) gene is associated with resistance to sulfa/sulfone drugs in Pneumocystis jirovecii. We investigated the DHPS mutation rate in three groups of Iranian HIV-positive and HIV-negative patients by polymerase chain reaction-restricted fragment length polymorphism analysis. Furthermore, an association between P.

Vitrification of mouse preantral follicles versus slow freezing: Morphological and apoptosis evaluation.

The aim of this study was evaluation of survivability, maturation rate and apoptotic gene expression of preantral follicles after vitrification and slow freezing technique. Normal mouse preantral follicles were randomly divided into three experimental groups. In the control group, follicles were cultured immediately; in the vitrification and slow freezing groups, follicles were cultured after vitrification-warming and slow freezing-thawing procedures.

Typing of Pneumocystis jirovecii isolates from Iranian immunosuppressed patients based on the Internal Transcribed Spacer (ITS) region of the rRNA gene.

Since there have been no published molecular studies of Pneumocystis jirovecii isolates from Iranian patients, we investigated the genotypes of such isolates recovered from HIV-infected patients, those undergoing cancer chemotherapy and patients with chronic obstructive pulmonary disease (COPD). P. jirovecii typing, based on ITS1 and ITS2 sequence analysis, was performed on 34 isolates from Iranian immunosuppressed patients.