Molecular characterization, phylogenetic and variation analyses of SARS-CoV-2 strains in India.

Unlabelled: In the wake of the havoc caused by the COVID-19 pandemic, it is imperative to use the available genomic sequence data to gain insight into the mutational and genomic diversity of SARS-CoV-2. Here we have performed comparative phylogenetic, mutational and genetic diversity analysis on 1962 SARS-CoV-2 genome sequences from seven worst hit Indian states during the third Covid-19 wave, to determine the SARS-CoV-2 strains and mutations in circulation during the third wave and the transmission pattern and disease epidemiology across the states and gain valuable insight into the viral evolution. 6083 Single nucleotide polymorphisms (SNPs) were discovered in the analysis with 93 SNPs common to all states.

Regenerative therapy in experimental parkinsonism: mixed population of differentiated mouse embryonic stem cells, rather than magnetically sorted and enriched dopaminergic cells provide neuroprotection.

Aim: The objective of the study was to develop regenerative therapy by transplanting varied populations of dopaminergic neurons, differentiated from mouse embryonic stem cells (mES) in the striatum for correcting experimental parkinsonism in rats.

Methods: mES differentiated by default for 7 days in serum-free media (7D), or by enhanced differentiation of 7D in retinoic acid (7R), or dopaminergic neurons enriched by manual magnetic sorting from 7D (SSEA-) were characterized and transplanted in the ipsilateral striatum of 6-hydroxydopamine-induced hemiparkinsonian rats. Neurochemical, neuronal, glial and neurobehavioral recoveries were examined.

A filtration method for rapid preparation of conjugates for immunoassay.

Modification of protein and other biopolymers by labeling them with small or macromolecules has become a very powerful research tool in biochemistry, molecular biology, diagnostics, and therapeutics. However, current methodologies available for their preparations are not straightforward and take several hours of incubation time. In this paper, we describe a new filtration-assisted technique for covalent conjugation between the reactive functional groups of two different molecules (small or macromolecules).