Influence of polymer structure on electroosmotic flow and separation efficiency in successive multiple ionic layer coatings for microchip electrophoresis.

The effect of successive multiple ionic layer (SMIL) coatings on the velocity and direction of EOF and the separation efficiency for PDMS electrophoresis microchips was studied using different polymer structures and deposition conditions. To date, the majority of SMIL studies have used traditional CE and fused-silica capillaries. EOF was measured as a function of polymer structure and number of layers, in one case using the same anionic polymer and varying the cationic polymer and in the second case using the same cationic polymer and varying the anionic polymer.

Measuring reaction rates on single particles in a microfluidic device.

The development of a simple method to measure reaction rates using magnetic microparticles in a microfluidic device is explored.

Detection of cardiac biomarkers using micellar electrokinetic chromatography and a cleavable tag immunoassay.

Biomarkers provide clinicians with an important tool for disease assessment. Many different biomarkers have been discovered, but few of them suffice as stand-alone indicators for disease presence or prognosis. Because no single biomarker can be relied upon for accurate disease detection there has been a substantial push for new multianalyte screening methods.

Generation of hydrophilic poly(dimethylsiloxane) for high-performance microchip electrophoresis.

Poly(dimethylsiloxane) (PDMS) has become one of the most widely used materials for microchip capillary electrophoresis and microfluidics. The popularity of this material is the result of its low cost, simple fabrication, and rugged elastomeric properties. The hydrophobic nature of PDMS, however, limits its applicability for microchip CE, microfluidic patterning, and other nonelectrophoresis applications.

Multi-analyte immunoassay using cleavable tags and microchip micellular electrokinetic chromatography.

A new multi-analyte immunoassay designed to screen for several biomarkers using cleavable tag chemistry and micellular electrokinetic chromatography was evaluated.