Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.

Background: Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.

Objective: Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.

Methods: Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).