Coupled catalytic states and the role of metal coordination in Cas9.

Controlling the activity of the CRISPR-Cas9 system is essential to its safe adoption for clinical and research applications. Although the conformational dynamics of Cas9 are known to control its enzymatic activity, details of how Cas9 influences the catalytic processes at both nuclease domains remain elusive. Here we report five cryo-electron microscopy structures of the active Cas9 complex along the reaction path at 2.

Scratching the Surface: Passivating Perovskite Nanocrystals for Future Device Integration.

Metal halide perovskite materials have recently upended the field of photovoltaics and are aiming to make waves across a multitude of other fields and applications. Recently, perovskite nanocrystals have been synthesized and are rapidly outpacing traditional semiconductor nanocrystals in application driven fields due to their inherent defect tolerance and facile tunability, resulting in high photoluminescent quantum yields and efficient devices. Future improvements to perovskite nanocrystals toward device driven applications must come at the perovskite surface.

The Effects of an Auditory Matching iPad App on Three Preschoolers' Echoic and Listener Responses.

We investigated the effects of an auditory match-to-sample protocol on three preschoolers' accurate echoics to 100 English words and advanced listener responses. The protocol was presented by using an iPad app Sounds the same: an app to target listening and speaking clearly. We used a combination of a multiple probe design (for echoic responses) and a delayed multiple probe design (for advanced listener literacy responses) with a time-lagged baseline across participants to test the effectiveness of the protocol.