Interferometric laser imaging for respiratory droplets sizing.

Due to its importance in airborne disease transmission, especially because of the COVID-19 pandemic, much attention has recently been devoted by the scientific community to the analysis of dispersion of particle-laden air clouds ejected by humans during different respiratory activities. In spite of that, a lack of knowledge is still present particularly with regard to the velocity of the emitted particles, which could differ considerably from that of the air phase. The velocity of the particles is also expected to vary with their size.

Automatic numerical focus plane estimation in digital holographic microscopy using calibration beads.

We present a new method to achieve autofocus in digital holographic microscopy. The method is based on inserting calibrated objects into a sample placed on a slide. Reconstructing a hologram using the inverse problems approach makes it possible to precisely locate and measure the inserted objects and thereby derive the slide plane location.

Novel Detection and Restorative Levodopa Treatment for Preclinical Diabetic Retinopathy.

Diabetic retinopathy (DR) is diagnosed clinically by directly viewing retinal vascular changes during ophthalmoscopy or through fundus photographs. However, electroretinography (ERG) studies in humans and rodents have revealed that retinal dysfunction is demonstrable prior to the development of visible vascular defects. Specifically, delays in dark-adapted ERG oscillatory potential (OP) implicit times in response to dim-flash stimuli (<-1.

Validation of a Head-mounted Virtual Reality Visual Field Screening Device.

Unlabelled: PRéCIS:: The C3 fields analyzer (CFA) is a moderately reliable perimeter preferred by patients to standard perimetry. While it does not approximate the gold standard, it was sensitive and specific for clinically defined glaucoma (area under the receiving operator characteristic curve=0.77 to 0.

Controlling fibroblast adhesion and proliferation by 1D AlO nanostructures.

The fibrotic encapsulation, which is mainly accompanied by an excessive proliferation of fibroblasts, is an undesired phenomenon after the implantation of various medical devices. Beside the surface chemistry, the topography plays also a major role in the fibroblast-surface interaction. In the present study, one-dimensional aluminium oxide (1D AlO) nanostructures with different distribution densities were prepared to reveal the response of human fibroblasts to the surface topography.

Reconstruction of in-line holograms: combining model-based and regularized inversion.

In-line digital holography is a simple yet powerful tool to image absorbing and/or phase objects. Nevertheless, the loss of the phase of the complex wavefront on the sensor can be critical in the reconstruction process. The simplicity of the setup must thus be counterbalanced by dedicated reconstruction algorithms, such as inverse approaches, in order to retrieve the object from its hologram.

Low-Intensity Exercise in Mice Is Sufficient to Protect Retinal Function During Light-Induced Retinal Degeneration.

Purpose: We previously reported that a specific treadmill running exercise regimen protects against light-induced retinal degeneration (LIRD) in mice. We hypothesized that this protective effect varies with running intensity. To test this, mice undergoing LIRD were run at different treadmill speeds and retinal function was assessed.

Faster emergence behavior from ketamine/xylazine anesthesia with atipamezole versus yohimbine.

Recent interest in reversal of the hypnotic effects of anesthesia has mainly focused on overcoming a surge in GABA-mediated inhibitory signaling through activation of subcortical arousal circuits or antagonizing GABA receptors. Here we examine the reversal of anesthesia produced from non-GABA agents ketamine/xylazine and the effects of antagonists of adrenoreceptors. These antagonists vary in selectivity and produce temporally unique waking behavior post-anesthesia.

Regularized reconstruction of absorbing and phase objects from a single in-line hologram, application to fluid mechanics and micro-biology.

Reconstruction of phase objects is a central problem in digital holography, whose various applications include microscopy, biomedical imaging, and fluid mechanics. Starting from a single in-line hologram, there is no direct way to recover the phase of the diffracted wave in the hologram plane. The reconstruction of absorbing and phase objects therefore requires the inversion of the non-linear hologram formation model.

TrkB signalling pathway mediates the protective effects of exercise in the diabetic rat retina.

Diabetic retinopathy is a leading cause of vision loss. Treatment options for early retinopathy are sparse. Exercise protects dying photoreceptors in models of retinal degeneration, thereby preserving vision.

Dopamine Deficiency Mediates Early Rod-Driven Inner Retinal Dysfunction in Diabetic Mice.

Purpose: Electroretinograms (ERGs) are abnormal in diabetic retinas before the appearance of vascular lesions, providing a possible biomarker for diabetic vision loss. Previously, we reported that decreased retinal dopamine (DA) levels in diabetic rodents contributed to early visual and retinal dysfunction. In the current study, we examined whether oscillatory potentials (OPs) could serve as a potential marker for detecting early inner retinal dysfunction due to retinal DA deficiency.

In-line particle holography with an astigmatic beam: setup self-calibration using an "inverse problems" approach.

The use of digital in-line holography for the characterization of confined flows in cylindrical geometry confinements (e.g., cylindrical pipe or cylindrical capillaries) is discussed.

Assessment and treatment of the risk of psychosis in adolescents--a review.

Background: When psychosis first presents, and particularly in the case of schizophrenia, the guidelines recommend rapid institution of treatment with atypical antipsychotics. Two different clinical pictures can be observed: psychoses with acute onset and those with insidious onset. Acute cases (60% of the total) have a favourable course in 85% of young patients but where onset is insidious and the symptoms are predominantly negative, the course is poor in 25% of subjects.

Accurate 3D tracking and size measurement of evaporating droplets using in-line digital holography and "inverse problems" reconstruction approach.

Digital in-line holography was used to study a fast dynamic 3D phenomenon: the evaporation of free-falling diethyl ether droplets. We describe an unsupervised reconstruction algorithm based on an "inverse problems" approach previously developed by our team to accurately reconstruct 3D trajectories and to estimate the droplets' size in a field of view of 7 × 11 × 20 mm 3. A first experiment with non-evaporating droplets established that the radius estimates were accurate to better than 0.

Evaporating droplet hologram simulation for digital in-line holography setup with divergent beam.

Generalized Lorenz-Mie theory (GLMT) for a multilayered sphere is used to simulate holograms produced by evaporating spherical droplets with refractive index gradient in the surrounding air/vapor mixture. Simulated holograms provide a physical interpretation of experimental holograms produced by evaporating Diethyl Ether droplets with diameter in the order of 50 μm and recorded in a digital in-line holography configuration with a divergent beam. Refractive index gradients in the surrounding medium lead to a modification of the center part of the droplet holograms, where the first fringe is unusually bright.

Long time exposure digital in-line holography for 3-D particle trajectography.

One advantage of digital in-line holography is the ability for a user to know the 3-D location of a moving particle recorded at a given time. When the time exposure is much larger than the time required for grabbing the particle image at a given location, the diffraction pattern is spread along the trajectory of this particle. This can be seen as a convolution between the diffraction pattern and a blurring function resulting from the motion of the particle during the camera exposure.

Size measurement of bubbles in a cavitation tunnel by digital in-line holography.

Digital in-line holography (DIH) with a divergent beam is used to measure size and concentration of cavitation bubbles (6-100 μm) in hydrodynamic facilities. A sampling probe is directly inserted in the cavitation tunnel, and the holograms of the bubbles are recorded through a transparent test section specially designed for DIH measurements. The recording beam coming from a fiber-coupled laser diode illuminates the sample volume, and holograms are recorded by a CMOS camera.

Adolescents and young adults at ultrahigh risk of psychosis: detection, prediction and treatment. A review of current knowledge.

Background: The onset of psychosis is mostly preceded by a prodromal phase in which occur a series of various attenuated positive symptoms, negative symptoms and functional impairment. Recently, several longitudinal studies have evaluated the validity and prediction power of the "prodromal risk syndrome for psychosis", which could lead to a better focused management of the patients at real risk of developing schizophrenia. At the same time, several authors have studied the pharmacological and non-pharmacological interventions for people at ultrahigh risk.

Time gate, optical layout, and wavelength effects on ballistic imaging.

A method to distinguish a hidden object from a perturbing environment is to use an ultrashort femtosecond pulse of light and a time-resolved detection. To separate ballistic light containing information on a hidden object from multiscattered light coming from the surrounding environment that scrambles the signal, an optical Kerr gate can be used. It consists of a carbon disulfide (CS(2)) cell in which birefringence is optically induced.

Ultrashort pulse propagation through a strongly scattering medium: simulation and experiments.

Forward light scattering of femtosecond pulses through strongly scattering media is investigated experimentally and numerically. Computations are based on a semi-Monte Carlo method including polarization effects when experiments depend on a Ti:sapphire regenerative amplifier (100 fs, 1 kHz, 1 mJ@ 800 nm). The temporal separation between ballistic light and scattered light is exhibited and used to perform optical depth measurements up to 22 (transmission factor of approximately 10(-10)).

Scattering of laser pulses (plane wave and focused gaussian beam) by spheres.

The scattering of laser pulses (in the femtosecond-picosecond range) by large spheres is investigated. We call a sphere large when its diameter is larger than the length associated with the pulse duration, allowing one to observe the temporal separation of scattering modes including surface waves.

Localized approximation for gaussian beams in elliptical cylinder coordinates.

We establish a localized approximation to evaluate the beam-shape coefficients of a Gaussian beam in elliptical cylinder coordinates. As for the case of spherical coordinates and of circular cylinder coordinates, this approximation provides an efficient way to speed up computations within the framework of a generalized Lorenz-Mie theory for elliptical cylinders.

Cylindrical localized approximation to speed up computations for Gaussian beams in the generalized Lorenz-Mie theory for cylinders, with arbitrary location and orientation of the scatterer.

A cylindrical localized approximation to speed up numerical computations in generalized Lorenz-Mie theory for cylinders, in a special case of perpendicular illumination, was recently introduced and rigorously justified. We generalize this approximation to the case when the cylinder is arbitrarily located and arbitrarily oriented in a Gaussian beam.

Scattering of a gaussian beam by an infinite cylinder with arbitrary location and arbitrary orientation: numerical results.

We present numerical results concerning the properties of the electromagnetic field scattered by an infinite circular cylinder illuminated by a circular Gaussian beam. The cylinder is arbitrarily located and arbitrarily oriented with respect to the illuminating Gaussian beam. Numerical evaluations are provided within the framework of a rigorous electromagnetic theory, the generalized Lorenz-Mie theory, for infinite cylinders.