Gold/silver-catalyzed synthesis of functionalized indoles from -allyl-2-alkynylanilines and α-diazo compounds 1,3-allyl migration.

Gold-catalyzed synthesis of decorated indoles has been developed through carbene insertion into N-H bonds of -allyl-2-(aryl/alkyl ethynyl)anilines using α-diazo compounds followed by cyclization and concomitant 1,3 migration of allyl fragments. The developed protocol tackles the inherited challenge of direct C3 functionalization and eliminates the need for a tertiary aniline precursor for the 1,3-migration reaction. The applicability of this transformation is showcased through the practical synthesis of analogs of small drug-like and pharmaceutically relevant molecules such as ibuprofen, estradiol, menthol, and borneol, The mechanism is well supported by control experiments and isolation of the reaction intermediate.

Phyllodes Tumor, A Cytomorphologic Study of 17 Cases with Histologic Correlation.

Background & Objective: Phyllodes tumor (PT) is a rare fibroepithelial tumor of the breast exhibiting varied clinicopathologic behavior, ranging from benign to borderline to frankly malignant, based on the presence of infiltrative margins, stromal overgrowth, stromal atypia, cellularity, and mitotic activity. In this study, a detailed cytomorphological study of cases of PT with the clinical and histological correlation was performed.

Methods: A cytomorphological study of 17 cases of histologically proven PT diagnosed between Jan 2014 and July 2021 was done retrospectively.

Lateral Positioning and Airway Management in Penetrating Abdominal Trauma: A Case Report.

Airway management in trauma patients is a critical component of resuscitation, often complicated by the unique positioning required by the nature and location of injuries. Penetrating abdominal trauma requiring lateral positioning presents an uncommon and challenging scenario for airway stabilization, particularly when traditional supine approaches are contraindicated. We describe the case of an 18-year-old male patient who was taken to the operating theater with a penetrating abdominal injury necessitating lateral positioning due to ongoing bleeding and hemodynamic instability.

Sulphur-decorated TiC MXene structures as high-capacity electrode for Zn-ion batteries: a DFT study.

MXene-based structures have gained tremendous attention in energy storage applications, especially in ion batteries due to their promising electrical conductivity and high energy storage properties. Herein, we studied sulphur-decorated TiC MXene structures for Zn-ion batteries with augmented storage capacity (462.5 mAh g).

Single-cell spatial immune profiling for precision immunotherapy in Lynch syndrome.

Lynch syndrome (LS) is the most common hereditary colorectal cancer (CRC) predisposition syndrome, characterized by a high mutational burden and microsatellite instability-high (MSI-H) tumors. Immunology of LS-associated CRC (LS-CRC) is unique, with significant implications for treatment. Despite well-established knowledge of LS immunology, immunotherapy dose and treatment response can vary significantly based on local tumor immunity and specific germline pathogenic variant of LS genes.

Validation of stay-green and stem reserve mobilization QTLs: physiological and gene expression approach.

Introduction: Abiotic stress significantly reduces the wheat yield by hindering several physiological processes in plant. Stay-green (SG) and stem reserve mobilization (SRM) are the two key physiological traits, which can contribute significantly to grain filling during stress period. Validation of genomic regions linked to SG and SRM is needed for its subsequent use in marker-assisted selection in breeding program.

Comparative evaluation of P-POSSUM and NELA scores in predicting 30-day mortality following emergency laparotomy: A prospective observational study.

Objective: Emergency laparotomy carries a 10-18% mortality risk, influenced by factors such as age, medical conditions, and sarcopenia. Scoring models like the Portsmouth physiological and operative severity score (P-POSSUM) and the National Emergency Laparotomy Audit (NELA) have been developed to predict outcomes and assist decision-making. Both models are widely used, but their effectiveness in predicting outcomes, particularly in the Indian context, requires further evaluation.

Pain out of plane: Lumbar erector spinae block redefining analgesia for acute soft tissue injury in emergency department.

Introduction: Soft tissue injuries resulting from trauma (motor vehicle collisions, falls, etc.) are frequently present to the Emergency Department. While opioid analgesia is commonly used, its side effects and dependence potential underscore the need for alternative approaches (1).

COVID-19 Pandemic-Related Perceived Stress, Insomnia, Depression, and Anxiety Among Rural Primary Care Health Workers: A Mediation Analysis.

Mental health of health care workers (HCWs) was affected during the COVID-19 pandemic due to direct handling of suspected and confirmed cases. While neurobiological mechanisms that mediate stress, depression, and anxiety are well established, psychological mechanisms are not. To assess (1) the prevalence of anxiety, depression, insomnia, and perceived stress among accredited social health activists, multipurpose health workers, auxiliary nurse midwives, and other certified HCWs of rural areas of Telangana, India and (2) the factors that mediate stress with depression and anxiety.

In-vitro Dilution of Human Plasma to Reduce ABO Antibody Titer Levels While Preserving Coagulation Factors.

The objective of this study was to prepare universal plasma by mixing Group A and Group B plasma with normal saline in varying proportions. This prospective in vitro study involved mixing blood group A and B plasma in ratios of 1:1, 3:1, 1:3, and with normal saline in ratios of 1:1:1, 2:2:1, and 1:1:2. The titration of IgG and IgM levels of anti-A and anti-B antibodies, as well as fibrinogen and factor VIII levels, were performed.

Low titre of agroinoculum with prolonged incubation period and low auxin concentration in the regeneration media are the key to high frequency of transformation in climate-resilient -type rice genotype Nagina 22.

Unlabelled: Developing an efficient and reproducible regeneration protocol holds paramount significance for advancing genetic transformation technologies in rice, facilitating their utilisation in crop improvement. Nagina 22 (N22), a climate-resilient -type rice genotype known for its tolerance against multiple stresses, lacks a standardised transformation protocol, limiting its utilisation as a background for genetic transformation. This study reports, for the first time, a highly efficient transformation and regeneration protocol for N22 using a CRISPR/Cas9 vector.

Developing anti-TDE vaccine for sensitizing cancer cells to treatment and metastasis control.

Tumor-derived exosomes (TDEs) mediate oncogenic communication, which modifies target cells to reinforce a tumor-promoting microenvironment. TDEs support cancer progression by suppressing anti-tumor immune responses, promoting metastasis, and conferring drug resistance. Thus, targeting TDEs could improve the efficacy of anti-cancer treatments and control metastasis.

Severity grading of hypertensive retinopathy using hybrid deep learning architecture.

Background And Objectives: Hypertensive Retinopathy (HR) is a retinal manifestation resulting from persistently elevated blood pressure. Severity grading of HR is essential for patient risk stratification, effective management, progression monitoring, timely intervention, and minimizing the risk of vision impairment. Computer-aided diagnosis and artificial intelligence (AI) systems play vital roles in the diagnosis and grading of HR.

Increasing Productivity and Recovering Nutritional, Organoleptic, and Nutraceutical Qualities of Major Vegetable Crops for Better Dietetics.

The intensive use of chemical fertilizers for vegetable cultivation to achieve higher productivity causes soil degradation, resulting in an alarming decline (25-50%) in nutritional quality and a reduction in a wide variety of nutritionally essential minerals and nutraceutical compounds in high-yielding vegetable crops over the last few decades. To restore the physio-chemical and biological qualities of soil as well as the nutritional and nutraceutical qualities of fresh produce, there is a growing desire to investigate the remedial impacts of organic sources of nutrition. This study specifically focused on the impact of six different ratios of chemical fertilizers and organic sources with microbial inoculation on vegetable productivity, nutrition quality, and soil health parameters.

Surgical site infection following incisional negative pressure wound therapy in lower limb amputation closure: A randomized controlled trial.

Background: Major amputations are a standard procedure being done for various etiologies of the lower limb. Surgical site infections often complicate the postoperative outcome of patients. Negative pressure wound therapy has evolved to have a preventive role in SSI.

Extracellular matrix re-normalization to improve cold tumor penetration by oncolytic viruses.

Immunologically inert or cold tumors pose a substantial challenge to the effectiveness of immunotherapy. The use of oncolytic viruses (OVs) to induce immunogenic cell death (ICD) in tumor cells is a well-established strategy for initiating the cancer immunity cycle (CIC). This process promotes the trafficking and infiltration of CD8+ T cells into tumors, thereby eliciting a tumor-specific immune response.

Implementing an inclusive digital health ecosystem for healthy aging: a case study on project SingaporeWALK.

Objective: To pilot a digital health technologies ecosystem known as project SingaporeWALK (earables and pps for ommunity iving and nowledge) that build capacity in older adults, senior center managers, health coaches, and caregivers in using health technologies (eg, wearables, apps, exergames) collaboratively in a gamified way for active aging.

Materials And Methods: The SingaporeWALK ecosystem was set up through 3 initiatives: (1) co-developing technologies with stakeholders; (2) raising digital literacy and capacity building; and (3) cultivating community and intergenerational bonding for active aging through gamified technology use.

Results: Significant improvements in older adults' self-reported physical and mental health post-intervention were observed.

Intraglandular botulinum toxin in postoperative head and neck fistula: a retrospective analysis.

Purpose: Orocervical (OCF) or pharyngocutaneous fistula (PCF) are one of the disastrous complications of head and neck cancer surgery. Conventional standards of management are predominantly conservative. Though a majority of such patients respond to conservative management, it nevertheless causes significant delay in wound healing.

ACSL4-mediated H3K9 and H3K27 hyperacetylation upregulates SNAIL to drive TNBC metastasis.

Triple-negative breast cancer (TNBC) has profound unmet medical need globally for its devastating clinical outcome associated with rapid metastasis and lack of targeted therapies. Recently, lipid metabolic reprogramming especially fatty acid oxidation (FAO) has emerged as a major driver of breast cancer metastasis. Analyzing the expression of major FAO regulatory genes in breast cancer, we found selective overexpression of acyl-CoA synthetase 4 (ACSL4) in TNBC, which is primarily attributed to the absence of progesterone receptor.

Expression and Immunolocalization of Aquaporins in the Buffalo Liver and Adipose Tissue.

Increasing incidences of fatty liver in humans and animals worldwide is the leading cause of liver related morbidities. Currently, in the face of the growing global increase in fatty liver, and the necessity to explore new factors significantly affecting it, aquaporins (AQPs) have become the focus of interest for many researchers. AQPs are membrane integral proteins involved in the transport of water, glycerol and other small solutes.

Effect of scrotal support application on seroma formation following minimal access surgery for inguinal hernia: A randomised controlled trial.

Background: The incidence of seroma formation is high following laparoscopic surgery for an inguinal hernia. Literature has shown many intraoperative techniques to reduce post-operative seroma formation. The hypothesis was made that scrotal support may reduce seroma formation following laparoscopic or robotic hernia surgery.

Investigating the genomic and metabolic abilities of PGPR Pseudomonas fluorescens in promoting plant growth and fire blight management.

Pseudomonas fluorescens is commonly found in diverse environments and is well known for its metabolic and antagonistic properties. Despite its remarkable attributes, its potential role in promoting plant growth remains unexplored. This study examines these traits across 14 strains residing in diverse rhizosphere environments through pangenome and comparative genome analysis, alongside molecular docking studies against Erwinia amylovora to combat fire blight.

Green synthesized CaO decorated ternary CaO/g-CN/PVA nanocomposite modified glassy carbon electrode for enhanced electrochemical detection of caffeic acid.

A highly selective, sensitive caffeic acid (CA) detection based on calcium oxide nanoparticles (CaO NPs) derived from extract of Moringa oleifera leaves decorated graphitic carbon nitride covalently grafted poly vinyl alcohol (CaO/g-CN/PVA) nanocomposite modified glassy carbon electrode (GCE) was studied. A facile sonochemical method was adapted to synthesis nanomaterials and characterized by HR-TEM (High resolution transmission electron microscopy), FT-IR (Fourier transform infrared spectroscopy), XRD (X-ray diffraction), FE-SEM (Field emission scanning electron microscopy), EDX (Energy dispersive X-ray analysis), Mapping and BET (Brunauer-Emmett-Teller) analysis, and electrochemical techniques. The nanocomposite modified GCE exhibited an excellent catalytic performance to the oxidation of CA under optimized conditions owing to better electron transfer efficiency, conductivity and high surface area of the electrode material.

Acquaintance, attitude, and perceived barriers regarding scientific research publications among clinical nurses: a cross-sectional study at tertiary care hospitals in western Rajasthan.

Introduction: In today's era, conducting nursing research is crucial for the advancement of the nursing profession. Scientific publications in clinical research aim to improve patient care outcomes and foster a sense of importance for nurses within the healthcare team. However, clinical nurses often fall behind due to factors such as limited familiarity, attitudes toward research, and encountered barriers.