[Trabeculae and the intertrabelcular tissue during the contraction of the frog atrium at decreased temperatures and sodium ion concentrations in solution].

Data characterizing the kinetics of the contraction of trabeculae and the intertrabelcular tissue have been obtained. It has been concluded that trabeculae can act as conductors/transmitters of contraction across the myocardium and play a leading role in this process, while the functional role of the intertrabelcular tissue is secondary and more passive, and consists in the optimization and support of the contractile process.

[Diagnostics of genital herpesvirus infection in women with tubal-peritoneal infertility].

Aim: Optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [CMV]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (PCR) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation.

Materials And Methods: Sixty-three women with tubal-peritoneal infertility were included in the study. Immunoenzyme assay, PCR, and modified Griess method were used.

[Microflora of reproductive system in women with tubal-peritoneal sterility].

Aim: To study the composition of microflora of lower and upper parts of reproductive system in women with tubal-peritoneal sterility.

Materials And Methods: One hundred and forty-five women with tubal-peritoneal sterilitywere examined. Laparoscopy with biopsy of ovaries and endometrium with evaluation of specimens by histology and PCR was performed in all patients.

[Blood erythropoietin level in female reproductive age (can the reproductive tissue be the source of erythropoietin in blood?)].

Blood erythropoietin level was investigated in females of reproductive age, who had normal peripheral red blood findings. It was determined that blood erythropoietin level was 1.5-2 times higher (20.

Microphotometric dynamic analysis of the histochemical acetylcholinesterase reaction.

Dynamic analysis of the histochemical reaction of Karnovsky-Roots for acetylcholinesterase activity (AChE) is reported. Two methods were used. The first method was videography and densitometric analysis of frames from the film.

[Isolation of highly pathogenic avian influenza (HPAI) A/5H5N1 strains from wild birds in the epizootic outbreak on the Ubsu-Nur Lake (June 2006) and their incorporation to the Russian Federation State Collection of viruses (July 3, 2006)].

Virological and molecular genetic studies of the field material collected in the epicenter of epizooty with high mortality rates among the wild birds on the coast of the Ubsu-Nur lake (Republic of Tyva, 51 degrees NL, 93 degrees EL, June 2006) revealed the etiological role of highly pathogenic avian influenza (HPAI) H5N1. Seven HPAI/H5N1 strains were isolated from the tracheal/cloacal swabs of clinically healthy, ill and recently dead great-crested grebes (Podiceps cristatus), cormorants (Phalacrocorax carbo), balt-coots (Fulica atra), and common terns (Sterna hirundo) collected on June 24, 2006, and incorporated to the RF State Collection of Viruses (with the July 3, 2006 priority). Full-length genome nucleotide sequences were incorporated to the GenBank (with the July 23, 2006 priority) (DQ852600-DQ852607).

[Disturbances in immunological reactivity in women with tuboperitoneal sterility].

The role of immune disturbances on the systemic and local levels is evaluated and the immunological mechanisms of tuboperitoneal sterility as the basis for the development of the methods of immunocorrection is grounded.

Character of Immune Disturbances and Their Correction by Including the Interferon Inductor in Complex Therapy for Turboperitoneal Infertility Associated with Chlamydial Infection.

The character of immune disturbances was studied in women with tuboperitoneal infertility associated with a chlamydial infection. The immunological methods included a study of the population and subpopulation composition of peripheral blood lymphocytes (CD3, CD7, CD22, CD4, CD8, ratio CD4/CD8). The general hemolytic activity of the complement and main components of the complement (C1-C5), the level of IgA, IgM, IgG, the concentration of CIC, the content of IL-2 and its soluble receptor (sIL-2R) were determined in blood serum and in peritoneal fluid before and after treatment.

[Characteristics of the population and subpopulation composition of peripheral blood lymphocytes in noncomplicated pregnancy based on flow cytofluorometry data].

The population and subpopulation composition of peripheral blood lymphocytes of 33 women was studied in the dynamics of non-complicated pregnancy by the method of flow cytofluorometry with the use of equipment and reagents supplied by Becton Dickinson, Inc. This study revealed that the non-complicated course of pregnancy was characterized by the adequate reaction of the mother's immune system ensuring the physiological development of the fetus during pregnancy and timely labor.

[Etiology and systemic immunity disturbances in patients with tuboperitoneal infertility].

The etiological structure of the inflammatory diseases of genitals at different levels of the reproductive tract of women with tuboperitoneal sterility was studied. The study revealed the prevalence of cocci in the cervical canal, anaerobes in the endometrium and chlamydiae in the abdominal cavity (peritoneal fluid, the ovarian capsule, fimbriated ends), as well as the presence of mixed infection in 27% of the patients under study. The specific features of immunity in women with tuboperitoneal sterility were established, which determined the main trends of the subsequent pathogenetically oriented immunocorrection.

[Immunopathological responses in women with chronic inflammatory diseases of the uterus and appendages and their therapeutic correction].

A total of 145 women with chronic inflammatory diseases of uterus and appendages (IDUA) were examined. Bacterioscopy of smears and culture of vaginal, cervical canal and uterine cavity contents were performed in all patients. Direct immunofluorescence and PCR were used for detection of chlamydiae in scrapes and smears and in biopsy specimens taken from endometrium.

[The role of different microorganisms in the genesis of tubal-peritoneal infertility and in immunity].

The character of microflora, directly causing inflammatory process in tuboperitoneal infertility, was studied. The study revealed the prevalence of microbial associations, Chlamydia infection was detected in half of the women, and in 17% of the patients this infection was for the first time detected in the upper section of their reproductive apparatus, which was indicative of the role of Chlamydia infection in the occlusion of the uterine tubes. Opportunistic microflora persisting in persons with defective antimicrobial protection was found.

[The characteristics of local immunity in Chlamydia-associated chronic inflammatory diseases of the organs of the lesser pelvis in women].

Local immune reactions in 107 women with Chlamydia-associated chronic endometritis and salpingo-oophoritis were studied on endometrial biopsy specimens. Of these women, group 1 consisted 45 patients with Chlamydia-associated chronic endometritis and salpingo-oophoritis and group 2 consisted of 62 such patients having, in addition, bacterial vaginosis and candidiasis. Among associated bacteria, epidermal staphylococci, Escherichia coli and their combinations prevailed.

Direct participation of phospholipids in transmembrane K(+)-transport.

The correlation between the characteristics of K(+)-transport across the mitochondrial and bilayer lipid membranes formed form mitochondrial lipids has been demonstrated. It has been shown that different modes of K(+)-efflux activation in mitochondria result in the appearance of K(+)-transporting phospholipid forms. The experiments described in this work suggest that current fluctuations similar to those observed for biological channels can be registered in unmodified bilayer lipid membranes containing no protein components.

[Conditions for optimal production of active oxygen forms by rat polymorphonuclear leukocytes].

Using the procedure of lysis by ammonium chloride followed by differentiation centrifuging, the fraction of cells containing polymorphonuclear leucocytes was isolated from the whole rat blood to study the conditions of optimal production of oxygen forms was determined from the chemiluminescent cell response in the presence of luminol. It was shown that chemiluminescent cell response depends on the temperature, pH and osmolarity of the medium as well as on the presence of various concentrations of Ca2+ in the incubation medium. The maximal chemiluminescent response of polymorphonuclear leucocytes was observed at 35-37 degrees C, pH 7,6, osmolarity of 0,15 and Ca2+ concentration of 5-10 mM.

[The characteristics of immunological homeostasis in women with sterility of tuboperitoneal and mixed origins].

Immunologic homeostasis was studied in 272 women suffering from sterility of a tuboperitoneal and mixed origin. The detected drastic disorders of immunologic homeostasis were characterized by predominant disorders of primary immunity mechanisms (leukopenia, monocytopenia, lymphopenia, reduced concentrations of IgA and IgM, lysozyme and complement in the blood serum). Types II, III, and IV immunopathologic reactions to uterine tube tissue homologous antigen suggest their contribution to a chronic inflammatory process.

[The role of chlamydial infection in the genesis of tuboperitoneal sterility in women].

The incidence of chlamydial infection in women with tuboperitoneal sterility is 49.5%. Clinically inflammatory diseases of the pelvic organs in such an infection are characterized by a primary chronic course with frequent recurrences and involvement of the cervix uteri, urethra, internal genitals, with moderately manifest clinical symptoms.

[Diagnostic and prognostic possibilities of using the indicators of blood serum middle molecule and medium-molecular peptide levels in inflammatory diseases of the small-pelvic organs].

Analysis of the diagnostic and prognostic potentialities of measurements of blood serum medium molecules and medium-molecular peptides in women with inflammatory diseases of the small pelvis organs has been carried out, involving 60 patients and 10 healthy women. Medium molecule levels were estimated with the use of Gabrielyan and Lipatova's method, the level of medium-molecular peptides by the biuret method. The results recommend these measurements to be included in the complex of clinical laboratory investigations carried out in patients with pelvic inflammations for the early detection of purulent ++tubo-ovarian inflammations, endogenous intoxication, and a more rational treatment.

[A scale for the diagnosis of the degree of severity of late pregnancy toxicosis].

This paper presents a scale for the evaluation of severity of late toxemia using 15 clinical and laboratory indices as well as central and regional hemodynamic parameters obtained by whole-body rheography and poly-rheography. Interpretation of the severity of late toxemia involved findings of functional postural and pharmacological tests. The scale is recommended for its use in the obstetric practice.

[Changes in the strength of the beta-adrenoreceptor inhibiting mechanism on the eve of and during labor determined by the partusisten test].

Partusisten was examined for effects on uterine contractility in 28-36- or 38-42-week pregnant women and parturients. The agent was shown to result in inhibited uterine contractility in the pregnant and parturient females, the contractility was decreased before and particularly during labor, which was indicative of lower beta-adrenoreceptor inhibitory mechanism force.

[Endogenous beta-adrenomimetic substance as a constituent of the adrenergic beta receptor blocking mechanism].

In order to reveal endogenous beta-adrenomimetic in the blood the authors proposed the biological testing of the serum and plasma on the isolated myometrium of the rat. For this purpose obsidan was used as a beta-adrenoblocker. It was shown that endogenous beta-adrenomimetic different from adrenaline was present in the blood of males, nonpregnant and pregnant females and parturients.