MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.

MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed that the TIR domain of MAL (MAL) forms filaments in vitro and induces formation of crystalline higher-order assemblies of the MyD88 TIR domain (MyD88). These crystals are too small for conventional X-ray crystallography, but are ideally suited to structure determination by microcrystal electron diffraction (MicroED) and serial femtosecond crystallography (SFX).

Vaccinia Virus Immunomodulator A46: Destructive Interactions with MAL and MyD88 Shown by Negative-Stain Electron Microscopy.

Vaccinia virus A46 is an anti-inflammatory and non-anti-apoptotic, two-domain member of the poxviral Bcl-2-like protein family that inhibits the cellular innate immune response at the level of the Toll/interleukin-1 receptor (TIR) domain-containing TLR adaptor proteins MAL, MyD88, TRAM, and TRIF. The mechanism of interaction of A46 with its targets has remained unclear. The TIR domains of MAL and MyD88 have been shown to signal by forming filamentous assemblies.

Fast Hydrogenation and Dehydrogenation of Pt/Pd Bimetal Decorated over Nano-Structured Ag Islands Grown on Alumina Substrates.

This study reports the fast hydrogenation and dehydrogenation of ultra-thin discrete platinum/palladium (Pt/Pd) bimetal over nano-structured Ag islands grown on rough alumina substrate by a RF magnetron sputtering technique. The morphology of Ag nanoislands was optimized by RF magnetron sputtering and rapid thermal annealing process. Later, Pt/Pd bimetal (10/10) nm were deposited by RF magnetron sputtering on the nanostructured Ag islands.

Regulation of signaling by cooperative assembly formation in mammalian innate immunity signalosomes by molecular mimics.

Innate immunity pathways constitute the first line of defense against infections and cellular damage. An emerging concept in these pathways is that signaling involves the formation of finite (e.g.

Molecular tools in understanding the evolution of Vibrio cholerae.

Vibrio cholerae, the etiological agent of cholera, has been a scourge for centuries. Cholera remains a serious health threat for developing countries and has been responsible for millions of deaths globally over the past 200 years. Identification of V.

Kaposi's sarcoma: a computational approach through protein-protein interaction and gene regulatory networks analysis.

Interactomic data for Kaposi's Sarcoma Associated Herpes virus (KSHV)-the causative agent of vascular origin tumor called Kaposi's sarcoma-is relatively modest to date. The objective of this study was to assign functions to the previously uncharacterized ORFs in the virus using computational approaches and subsequently fit them to the host interactome landscape on protein, gene, and cellular level. On the basis of expression data, predicted RNA interference data, reported experimental data, and sequence based functional annotation we also tried to hypothesize the ORFs role in lytic and latent cycle during viral infection.