Grass carp is a nonnative freshwater fish in Bangladesh, and its seed quality is declining due to issues like inbreeding and negative selection, prompting the need for improved breeding techniques.
This study focused on optimizing sperm cryopreservation protocols to enhance the availability of quality grass carp seed, utilizing hormone-induced mature males from a broodstock developed from imported Chinese fingerlings.
The research found that specific conditions, such as using Alsever's solution with 10% DMSO, yielded high motility rates in sperm and viable fertilization and hatching rates from cryopreserved sperm; however, further improvements are needed in these rates for commercial hatchery success.
The study investigates the acute toxicity of Phenthoate 50 EC on common carp fingerlings, revealing a lethal concentration (LC) of 7.39 ppm over 96 hours.
Significant neurotoxic effects were observed, including a decrease in brain acetylcholinesterase activity and increased liver enzyme activities, indicating potential damage to the nervous system and liver.
Histopathological changes, along with alterations in blood, kidney, and gill morphology, suggest that exposure to Phenthoate leads to oxidative stress and biochemical changes, impacting the overall health of aquatic organisms.