Estimating water levels caused by a tropical storm along the Bangladesh coast: A numerical approach.

In this study, water levels resulting from the dynamic interaction of tide and surge are estimated by solving a 2-D vertically integrated shallow water equations numerically. To solve the equations on the specific 2-D grid, the explicit Leapfrog scheme is implemented, adopting a staggered Arakawa C-grid. The domain's complex land-sea interface is approximated through the stair-step method in order to employ the finite difference technique.

Loop-mediated isothermal amplification (LAMP) assay for specific and rapid detection of Dickeya fangzhongdai targeting a unique genomic region.

Dickeya fangzhongdai, a bacterial pathogen of taro (Colocasia esculenta), onion (Allium sp.), and several species in the orchid family (Orchidaceae) causes soft rot and bleeding canker diseases. No field-deployable diagnostic tool is available for specific detection of this pathogen in different plant tissues.

Development of a CAPS Marker and a LAMP Assay for Rapid Detection of Subsp. and Differentiation from Subsp. on Blueberry.

Bacterial leaf scorch (BLS), caused by (), is a prevalent disease of blueberries in the southeastern United States. Initially, this disease was reported to be caused by subsp. ().

Solving protoplanetary structure equations using Adomian decomposition method.

In this paper, the distribution of thermodynamic variables in the protoplanets formed by gravitational instability in the mass range ( Jupiter mass = gm) is investigated in their initial state by solving the structure equations via the Adomian decomposition method. Concerning the heat transfer in the protoplanets, the mode of convection is taken into account. The outcomes indicate that there is a reasonably good agreement between the Adomian semi-analytical solution containing only first 8 terms and the numerical results.

Genome-informed loop-mediated isothermal amplification assay for specific detection of Pectobacterium parmentieri in infected potato tissues and soil.

Pectobacterium parmentieri (formerly Pectobacterium wasabiae), which causes soft rot disease in potatoes, is a newly established species of pectinolytic bacteria within the family Pectobacteriaceae. Despite serious damage caused to the potato industry worldwide, no field-deployable diagnostic tests are available to detect the pathogen in plant samples. In this study, we aimed to develop a reliable, rapid, field-deployable loop-mediated isothermal amplification (LAMP) assay for the specific detection of P.

Lump, lump-stripe, and breather wave solutions to the (2 + 1)-dimensional Sawada-Kotera equation in fluid mechanics.

The present study investigates the lump, one-stripe, lump-stripe, and breather wave solutions to the (2+1)-dimensional Sawada-Kotera equation using the Hirota bilinear method. For lump and lump-stripe solutions, a quadratic polynomial function, and a quadratic polynomial function in conjunction with an exponential term are assumed for the unknown function giving the solution to the mentioned equation, respectively. On the other hand, only an exponential function is considered for one-stripe solutions.

f. sp. Molecular Diagnostics Past, Present and Future.

Watermelon is an important commercial crop in the Southeastern United States and around the world. However, production is significantly limited by biotic factors including fusarium wilt caused by the hemibiotrophic fungus forma specialis (Fon). Unfortunately, this disease has increased significantly in its presence over the last several decades as races have emerged which can overcome the available commercial resistance.

Molecular Characterization of Laboratory Mutants of f. sp. Resistant to Prothioconazole, a Demethylation Inhibitor (DMI) Fungicide.

f. sp. (FON) is the causal agent of Fusarium wilt in watermelon, an international growth-limiting pathogen of watermelon cultivation.

A Core35S Promoter of Cauliflower Mosaic Virus Drives More Efficient Replication of Turnip Crinkle Virus.

The 35S promoter with a duplicated enhancer (frequently referred to as 2X35S) is a strong dicotyledonous plant-specific promoter commonly used in generating transgenic plants to enable high-level expression of genes of interest. It is also used to drive the initiation of RNA virus replication from viral cDNA, with the consensus understanding that high levels of viral RNA production powered by 2X35S permit a more efficient initiation of virus replication. Here, we showed that the exact opposite is true.

Sensitivity of Isolates from Peanut Seeds in Georgia to Azoxystrobin, a Quinone outside Inhibitor (QoI) Fungicide.

infects peanuts and produces a mycotoxin called aflatoxin, a potent human carcinogen. In infected peanuts, it can also affect peanut seed quality by causing seed rot and reducing seed viability, resulting in low germination. In 2020, peanut seeds in Georgia had lower than expected germination and a high frequency of contamination.

Phylogenetic and phenotypic characterization of Fusarium oxysporum f. sp. niveum isolates from Florida-grown watermelon.

Fusarium wilt of watermelon (Citrullus lanatus) caused by Fusarium oxysporum f. sp. niveum (Fon), has become an increasing concern of farmers in the southeastern USA, especially in Florida.

Marker Development for Differentiation of f. sp. Race 3 from Races 1 and 2.

Fusarium wilt of watermelon, caused by f. sp. (FON), is pathogenic only to watermelon and has become one of the main limiting factors in watermelon production internationally.

Turfgrass Disease Diagnosis: Past, Present, and Future.

Turfgrass is a multibillion-dollar industry severely affected by plant pathogens including fungi, bacteria, viruses, and nematodes. Many of the diseases in turfgrass have similar signs and symptoms, making it difficult to diagnose the specific problem pathogen. Incorrect diagnosis leads to the delay of treatment and excessive use of chemicals.

First Report of Hop stunt viroid Infecting Citrus Trees in Georgia, USA.

In recent years, citrus production has rapidly increased within the state of Georgia (USA), and there are now citrus plantings within at least 32 counties in residential, production, and nursery settings. Among the pathogens capable of infecting citrus are viroids, the smallest plant pathogens. Viroids are comprised of circular, single-stranded RNA ranging from 246-463 nucleotides in length (Ito et al.

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification.

Phytophthora capsici is a devastating oomycete pathogen that affects many important solanaceous and cucurbit crops causing significant economic losses in vegetable production annually. Phytophthora capsici is soil-borne and a persistent problem in vegetable fields due to its long-lived survival structures (oospores and chlamydospores) that resist weathering and degradation. The main method of dispersal is through the production of zoospores, which are single-celled, flagellated spores that can swim through thin films of water present on surfaces or in water-filled soil pores and can accumulate in puddles and ponds.

Development of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of .

A loop-mediated isothermal amplification (LAMP) assay was developed for simple, rapid and efficient detection of (CuLCrV), one of the most important begomoviruses that infects cucurbits worldwide. A set of six specific primers targeting a total 240 nt sequence regions in the DNA A of CuLCrV were designed and synthesized for detection of CuLCrV from infected leaf tissues using real-time LAMP amplification with the Genie III system, which was further confirmed by gel electrophoresis and SYBR™ Green I DNA staining for visual observation. The optimum reaction temperature and time were determined, and no cross-reactivity was seen with other begomoviruses.

Characterization of Resistance to Major Tropical Root-Knot Nematodes ( spp.) in .

Root-knot nematodes ( spp.) are important contributors to yield reduction in tomato. Though resistant cultivars to common species (, , and ) are available, they are not effective against other major species of root-knot nematodes.

Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry.

Bacterial leaf scorch, caused by Xylella fastidiosa, is a major threat to blueberry production in the southeastern United States. Management of this devastating disease is challenging and often requires early detection of the pathogen to reduce major loss. There are several different molecular and serological detection methods available to identify the pathogen.

Conferring virus resistance in tomato by independent RNA silencing of three tomato homologs of Arabidopsis TOM1.

The TOM1/TOM3 genes from Arabidopsis are involved in the replication of tobamoviruses. Tomato homologs of these genes, LeTH1, LeTH2 and LeTH3, are known. In this study, we examined transgenic tomato lines where inverted repeats of either LeTH1, LeTH2 or LeTH3 were introduced by Agrobacterium.