Publications by authors named "Md Arif Sakil"
Article Synopsis
- Autophagy-defective mutants of Physcomitrium patens, specifically atg5 and atg7, display strong tolerance to desiccation and can survive exposure to high levels of hydrogen peroxide (H2O2) that are lethal to wild-type (WT) plants.
- The study reveals that autophagy in WT plants leads to increased cell death and vacuole collapse when exposed to H2O2, as higher levels of H2O2 are observed compared to the mutants.
- Further experiments suggest that autophagy-derived amino acids contribute to H2O2 production through mitochondrial and chloroplast electron transport chains, indicating that autophagy can promote cell death under stress conditions by generating reactive oxygen species.
Leaf senescence accompanied by yellowing and Rubisco degradation occurs prematurely in response to various stresses. However, signaling pathways between stress perception and senescence responses are not understood fully, although previous studies suggest the involvement of reactive oxygen species (ROS). While investigating the physiological functions of autophagy in Physcomitrium patens using wild-type (WT) and autophagy-deficient atg5 strains, we found that Physcomitrium colonies senesce prematurely under dark or nitrogen-deficient conditions, with atg5 senescing earlier than WT.
View Article and Find Full Text PDFThe physiological implications of autophagy in plant cells have not been fully elucidated. Therefore, we investigated the consequences of autophagy in the moss Physcomitrella by measuring biochemical parameters (fresh and dry weights; starch, amino acid, carbohydrate, and NH3 content) in wild-type (WT) and autophagy-deficient atg5 Physcomitrella cells. We found higher starch levels and a higher net starch synthesis rate in WT cells than in atg5 cells cultured in a glucose-containing culture medium, whereas net starch degradation was similar in the two strains cultured in a glucose-deficient culture medium.
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