Publications by authors named "Md Akkas Ali"
Spectral flow cytometry provides greater insights into cellular heterogeneity by simultaneous measurement of up to 50 markers. However, analyzing such high-dimensional (HD) data is complex through traditional manual gating strategy. To address this gap, we developed CAFE as an open-source Python-based web application with a graphical user interface.
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- Flow cytometry is a technique for analyzing immune cells, and spectral flow cytometry enhances this by allowing for high-dimensional analysis, addressing the limitations of traditional methods.
- A novel analysis pipeline was developed to better handle data from large antibody panels, helping to identify rare T cell populations in aging by applying advanced methods like dimensionality reduction and unsupervised clustering.
- The study compared T cell clusters from young and aged mice, revealing significant age-related differences in T cell subsets, and demonstrated the pipeline's effectiveness with human PBMC datasets, potentially paving the way for new therapeutic targets in age-related immune issues.
Flow cytometry is a widely used technique for immune cell analysis, offering insights into cell composition and function. Spectral flow cytometry allows for high-dimensional analysis of immune cells, overcoming limitations of conventional flow cytometry. However, analyzing data from large antibody panels can be challenging using traditional bi-axial gating strategies.
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- - Multiple system atrophy (MSA) is a rare, rapidly progressing neurodegenerative disease characterized by the presence of alpha-synuclein inclusions and results in severe brain damage, including demyelination and inflammation.
- - Researchers used a modified AAV to study the role of CD8+ T cells in MSA by creating a mouse model, revealing significant infiltration of these cells into the striatum after inducing MSA-related pathology.
- - Findings showed that while CD8+ T cells do not drive MSA progression, they play a role in modulating neuroinflammation and demyelination, which alters disease responses.
Mitochondria are cellular organelles critical for ATP production and are particularly relevant to cardiovascular diseases including heart failure, atherosclerosis, ischemia-reperfusion injury, and cardiomyopathies. With advancing age, even in the absence of clinical disease, mitochondrial homeostasis becomes disrupted (e.g.
View Article and Find Full Text PDFAging is a strong risk factor for atherosclerosis and induces accumulation of memory CD8 T cells in mice and humans. Biological changes that occur with aging lead to enhanced atherosclerosis, yet the role of aging on CD8 T cells during atherogenesis is unclear. In this study, using femle mice, we found that depletion of CD8 T cells attenuated atherogenesis in aged, but not young, animals.
View Article and Find Full Text PDFWater deficit is a major limiting condition for adaptation of maize in tropical environments. The aims of the current observations were to evaluate the kernel water relations for determining kernel developmental progress, rate, and duration of kernel filling, stem reserve mobilization in maize. In addition, canopy temperature, cell membrane stability, and anatomical adaptation under prolonged periods of pre- and post-anthesis water deficit in different hybrids was quantified to support observations related to kernel filling dynamics.
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