Circadian analysis of mononuclear cells in the rat following pinealectomy and superior cervical ganglionectomy.

The role of the pineal gland and adrenocorticosteroids in circadian rhythmicity of immune function was investigated in Sprague-Dawley rats that either had been pinealectomized (PX) or had undergone superior cervical ganglionectomy (SCGX), which functionally denervates the pineal. For both experiments, conducted between August and October, rats were entrained to a 10-h light:14-h dark cycle and fed ad libitum. The circadian rhythm in monocytes (ANOVA, p less than .

A circannual cycle in pinealocyte synaptic ribbons in the hibernating and seasonally reproductive 13-lined ground squirrel (Spermophilus tridecemlineatus).

Pinealocyte synaptic ribbons (SR) in the 13-lined ground squirrel were quantified at monthly intervals over a one-year period spanning the hibernating and reproductive cycles of these animals. SR numbers were high (70-90/20,000 microns2) during periods of activity and reproductive quiescence (May-October). With the onset of hibernation (November-December) there was a rapid 6 to 7-fold reduction in SR frequency.

Ultrastructure and organization of circumventricular organs and endolymphatic tubules in the treefrog, Hyla versicolor.

The morphology and anatomical relationship of the paraphysis, anterior choroid plexus, and endolymphatic tubules of Hyla versicolor suggest that these structures may act as a functional unit to regulate composition of the cerebrospinal fluid (CSF). Ependymal cells of both the paraphysis and the choroid plexus exhibit ultrastructural features (an extensive microvillar border, large intercellular spaces containing an amorphous material, and apically located mitochondria) which indicate active exchange between the blood and CSF. Mast cells (which may play a role in regulating vascular permeability) also occur on the brush border.

Magnetic resonance imaging (MRI) effects on rat pineal neuroendocrine function.

This study investigated the effects of MRI on receptor-mediated activation of pineal gland indole biosynthesis. Exposure of rats to MRI reduced the effects of isoproterenol on pineal serotonin and N-acetylserotonin levels suggesting that strong magnetic fields and/or radio-frequency pulsing used in MRI inhibited beta-adrenergic activation of the gland. There was no effect of MRI on saline controls.

Day-night differences in the vesicle populations of nerve terminals in the rat and chick pineal gland.

Day-night differences in the ultrastructure of pineal neuronal elements were quantitated in the rat and the chick--two species that exhibit important differences in adrenergic control of melatonin synthesis. Area densities of small clear vesicles (40-60 nm) and small dense-cored vesicles (40-60 nm) in nerve terminals were significantly reduced during the night in the rat. In the chick, there were no day-night differences in the frequency of clear vesicles, but the number of small dense-cored vesicles tended to be greater at night.

Pineal gland free amino acids and indoles during postnatal development of the rat: correlations in individual glands.

Free amino acids and indoles were measured by HPLC in single pineal glands of 5-, 10- and 20-day-old rats sacrificed during mid-light and mid-dark at each age. Melatonin was detectable in neonates (5-day-old), but day vs night differences in indole constituents did not occur until 10 days of age. Free amino acid steady state levels were high in neonates and there was a tendency for reversal of day vs night differences coinciding with the onset of circadian rhythmicity in indole biosynthesis.

Monolobar Caroli's disease.

Two cases of Caroli's disease confined to the left lobe of the liver are described. One patient is a 13-yr-old who presented with acute pancreatitis. The second patient presented with obstructive jaundice at age 60.

Population-based epidemiology of human immunodeficiency virus infection in Western Australia. Western Australian AIDS Advisory Committee.

A total of 328 cases of infection with human immunodeficiency virus (HIV) in Western Australia in 1983-1987 was studied with respect to demographic factors, the risk profile, the clinical progression of disease, the utilization of inpatient services and trends in incidence over time. The crude incidence rates were 8.8 cases/100,000 person-years in men and 0.

The study of caries prevalence in children in a developing country.

This study has shown that prevalence of dental caries in a rural section of a developing country is related to age, socioeconomic status, and specific location. Caries prevalence is also directly correlated with family consumption of sugar. No correlation could be found, however, with regard to sex.

A seasonal pineal peptide rhythm persists in superior cervical ganglionectomized rats.

Neurohypophyseal peptide hormone activity is present in the pineal gland of mammals, and varies over a seasonal cycle. Pineal peptide levels, measured by arginine vasotocin (AVT) radioimmunoassay, increase dramatically for a brief time during August each year. The manner in which this cycle is regulated is as yet unknown.

Effects of isoproterenol on synaptic ribbons in pinealocytes of the rat and C57BL/6J mouse.

Synaptic ribbons (SR) in melatonin-deficient pinealocytes of the C57BL/6J mouse were quantitatively compared to SR in pinealocytes of the rat after beta-adrenergic receptor activation by isoproterenol. Two populations of SR comprising synaptic spherules (SRsp) and synaptic rods (SRr) were described in both the mouse and the rat, but species differences existed in the ratio of SRr to SRsp. Isoproterenol caused a significant increase in frequency of SR of the rat but had little or no effect on SR populations in the mouse.

Ultrastructure and biochemistry of the pineal organ in deep-sea lanternfishes (Myctophidae).

Pineal structural and biochemical adaptations in lanternfishes included: 1) few photoreceptor outer segment discs; 2) conventional synapses between photoreceptors and pineal neurons; and 3) low levels (0-60 pg/pineal) of serotonin compared to those (greater than 1.0 ng/pineal) in the goldfish pineal organ. These findings suggest reduced photosensory and/or neuroendocrine functions in these deep-sea fishes.

Day-night variability in acid phosphatase activity of pineal gland macrophages.

Acid phosphatase (ACP) activity was investigated in the photoreceptive pineal gland of the goldfish by both light and electron microscopy. Intraluminal macrophages exhibited the greatest ACP reactivity that was generally confined to lysosomal bodies containing membranous debris. Quantitative light microscopic evaluation revealed significantly higher ACP reactivity in animals collected at mid-light compared to those collected at mid-dark suggesting that functional activities of macrophages in this photoneuroendocrine organ are cyclical over the 24-h photoperiod.

Variation in hospital morbidity in the male workforce of Western Australia.

The associations of hospital morbidity with occupation were studied in Western Australian men of working age in 1981-2. Data on hospital morbidity were derived from a population based system that covered all short stays in hospitals in the state. Occupations were grouped into 12 major categories and conditions were coded using the International Classification of Diseases.

Ultrastructural and biochemical responses of photoreceptor pinealocytes to light and dark in vivo and in vitro.

The teleost pineal organ contains functional photoreceptors that synapse with pinealofugal neurons. This study examined the effects of environmental lighting on protein content and levels of putative amino acid transmitters, as well as structural components associated with protein synthesis and neurotransmission. Goldfish subjected to continual illumination for 3 days tended to have increased pineal levels of free amino acids and protein compared to dark adapted glands.

Determinants of chronic bronchitis and lung dysfunction in Western Australian gold miners.

The relation of chronic bronchitis and respiratory dysfunction to age, tobacco smoking, and occupational exposure to surface and underground mining operations were examined in a cross sectional survey of 1363 men employed in the Kalgoorlie mining industry in 1985. Overall, the prevalence of chronic bronchitis was 14%. Eleven per cent of the workers had obstructive lung disorder (FEV1/FVC less than 0.

Electrophysiology and ultrastructure of eustachian ridge from cat right atrium: a comparison with SA node.

Intracellular microelectrode and electron microscopic techniques were used to investigate and correlate the electrophysiology of subsidiary pacemaker activity with the presence of cells having ultrastructural characteristics of pacemaker cells i.e. P cells, in Eustachian ridge tissue isolated from cat right atrium.

Pinealocyte dense-cored vesicles and synaptic ribbons: a correlative ultrastructural-biochemical investigation in rats and mice.

Dense-cored vesicles (DCV) and synaptic ribbons (SR) were quantified in the pineal gland of the rat (Sprague-Dawley) and mouse (Sasco/ICR strain), and day/night differences in frequency of these organelles correlated with levels of indoles determined by high performance liquid chromatography (HPLC). There were significant day/night differences in levels of serotonin (5HT), 5-hydroxyindole acetic acid (5HIAA), N-acetyl-5HT, and melatonin in the rat gland. Melatonin and N-acetyl-5HT were not detectable in the mouse gland sampled every 4 h over the light:dark cycle.

Maternal ethanol consumption: lack of effect on synaptogenesis in layer I of the motor cortex in 19-day-old rat offspring.

Previous studies showed that in utero exposure to ethanol has an adverse effect on development of the central nervous system. Included among those abnormalities is the evidence that in utero exposure to ethanol alters the development of the cerebral cortex in terms of the arborization of pyramidal dendrites and the concentration of neurotransmitters and neurotransmitter receptors. However, limited information is available about the influence of in utero exposure to ethanol on synaptogenesis in the cortex.

Is hypertrophic osteoarthropathy really so rare in regional enteritis?

We describe a patient with hypertrophic osteoarthropathy secondary to regional enteritis, and review the literature on their association. The apparent different distribution of hypertrophic osteoarthropathy secondary to other causes may be artifactual and related to inadequate radiological investigation. Hypertrophic osteoarthropathy does not appear to be related to regional enteritis activity.

Correlations of serotonin and its metabolites in individual rat pineal glands over light:dark cycles and after acute light exposure.

Profiles of pineal indolealkylamines were estimated by high performance liquid chromatography and were correlated in individual glands of male rats sacrificed over several light:dark cycles and after acute exposure to light at night. A significant and positive correlation of 5HIAA vs 5HT in individual glands over both normal and experimental lighting conditions suggested that oxidative deamination is not a major factor in photic regulation of pineal 5HT levels and that the formation of 5HIAA is dependent on substrate availability. Regression analysis of other indole constituents revealed that there was a positive and significant correlation between 5HT vs N-acetylserotonin, but not between 5HT vs melatonin and N-acetylserotonin vs melatonin in individual glands during the dark phase of a light:dark cycle.

Uptake and metabolism of indole compounds by the goldfish pineal organ.

Indole metabolism was studied in the pineal organ of the goldfish by radioautography and high-performance liquid chromatography. The rate of uptake of tritiated serotonin was rapid in vitro with dense labeling over the photoreceptor cells. Tritiated tryptophan was taken up at a slower rate and the label was distributed evenly over the epithelium.