The effect of ethoxyquin on tissue peroxidation and immune status of single comb White Leghorn cockerels.

The responses to supplementing the diet of Single Comb White Leghorn (SCWL) cockerels with ethoxyquin were tested on two parameters: 1) tissue peroxidation and 2) immune response. In the first experiment, three concentrations of supplemental ethoxyquin (0, 500, and 1,000 ppm) were added to a basal diet and fed to SCWL cockerels for 6 wk. Tissue peroxidation was assessed by measuring the thiobarbituric acid reactive substances (TBARS) concentration in the liver, kidney, heart, and spleen.

Determination of carotenoid pigments, retinol, and alpha-tocopherol in feeds, tissues, and blood serum by normal phase high performance liquid chromatography.

A normal phase HPLC method employing a hexane:ethyl acetate mobile phase solvent gradient for the simultaneous separation and quantification of carotenoid pigments and vitamins A and E has been developed. The gradient consists of a 10-min linear change from 1 to 2 mL/min and 95:5 to 70:30 hexane:ethyl acetate mobile phase, which is followed by 7 min of isocratic mobile phase of 70:30 hexane:ethyl acetate at 2 mL/min. The stationary phase consisted of a 4-mu silica column.

alpha-tocopherol, beta-carotene, and retinol enrichment of chicken eggs.

Due to the numerous health benefits associated with consumption of antioxidants such as alpha-tocopherol and beta-carotene, an experiment was conducted to determine dietary levels that would significantly enhance their concentration in chicken egg yolks. In the experiment, 127 Single Comb White Leghorn laying hens were divided into treatment groups (n = 40 per treatment group) and fed diets containing 0, 50, 100, 200, or 400 mg/kg beta-carotene, dl-alpha-tocopheryl acetate, or their combination. Yolk alpha-tocopherol increased (P < .

Amylase levels in the tissues and body fluids of several primate species.

1. Serum amylase levels in the gorilla, orang-utan, chimpanzee and squirrel monkey are similar to man. Serum amylase in the Rhesus macaque is almost a whole order of magnitude higher than man.

Amylase levels in the tissues and body fluids of the domestic cat (Felis catus).

1. The amylase activities of serum, liver, pancreas, submaxillary and parotid glands, saliva, duodenum, colon, lung, heart, spleen, kidney and skeletal muscle of the domestic cat, were determined. 2.