A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

A rapid procedure involving DNA-cellulose chromatography followed either by sedimentation in a high-salt glycerol gradient or by gel filtration is described for the complete purification of Escherichia coli DNA-dependent RNA polymerase.

Inhibitors of kallikrein in human plasma.

Human plasma was fractionated by ammonium sulfate precipitation, DEAE-cellulose chromatography, and Sephadex G-200 gel filtration to determine which method would give the greatest number of clearly separable kallikrein inhibitory peaks. With G-200 gel filtration three peaks could be separated which were demonstrated to contain alpha(2)-macroglobulin, C1 inactivator, and alpha(1)-antitrypsin. No other kallikrein inhibitors could be identified.

Alpha-tocopherol in the retinal outer segment of bovine eyes.

α-Tocopherol was identified in lipid extracts of bovine retinal outer segment (ROS) preparations. Positive identification was obtained by the thin layer chromatographic characteristics of the tocopherol form and its oxidation product α-tocopherylquinone, and by the ultraviolet spectrum of the oxidized and KBH4-reduced form of the tocopherylquinone. In the ROS preparations used, α-tocopherol chromanol was the predominant species, the quinone form accounting for 25% or less of the total.

The isolation of human plasma prekallikrein.

1. The isolation of human plasma prekallikrein was achieved by fractionating human plasma on diethylaminoethyl cellulose (DEAE) in the presence of heparin.2.

Aluminum-rich apatite.

More than 25 atomic percent of aluminum can susbtitute for other cations in the structure of apatite [A(10)(XO(4)) (6)Z(2)]. Such a synthetic product was obtained by expelling volatile constituents (H(2)O and F) from morinite during thermal treatment. Infrared absorption spectra, chemical analysis, and x-ray powder diffraction demonstrate that the aluminum has two coordination numbers, and more than twice as much aluminum substitutes for calcium (A position) as for phosphorus (X position).

Biologic precipitation of fluorite.

X-ray diffraction patterns show that the statoliths of marine mysid crustaceans are composed of fluorite, and that this mineral is also a principal phase of the gizzard plates of some tectibranch gastropods. A phosphatic phase is also indicated by chemical analyses in the gizzard plates, but its crystallochemical characterization has not been feasible by x-ray diffraction. The occurrence of fluorite in mysid statoliths confirms the earlier interpretations based on insufficient documentation.