Uncompetitive inhibition by adenine of the RNA-N-glycosidase activity of ribosome-inactivating proteins.

Ricin is a member of the ribosome-inactivating protein (RIP) family with RNA-N-glycosidase activity which inactivates eukaryotic ribosomes by specifically removing adenine from the first adenosine of a highly conserved GAGA loop present in 28S rRNA. Free adenine protects ribosomes in cell-free systems from inactivation by ricin. Protection by adenine is highly specific, since AMP, adenosine and modified adenines (1-methyladenine and ethenoadenine) were completely ineffective.

The RNA-N-glycosidase activity of Shiga-like toxin I: kinetic parameters of the native and activated toxin.

Shiga toxin and Shiga-like toxins are ribosome-inactivating proteins with RNA-N-glycosidase activity which remove a specific adenine from 28S RNA. The toxins are composed of an A subunit non-covalently associated to a multimer of receptor-binding B subunits. Near the COOH-terminus of the A subunit, a disulfide-bonded loop contains two trypsin-sensitive arginine residues.

Prevalence of enteric adenovirus from acute gastroenteritis: a five year study.

Adenoviruses were detected in the stools in 459 of 3932 (11.6%) pediatric children hospitalized with acute gastroenteritis from January 1981 to December 1985. Out of the 459 adenovirus specimens 325 (8.

A two-year longitudinal study on the etiology of acute diarrhea in young children in Northern Italy.

During a 24-month period, 561 young children (6 months-2 years of age) hospitalized for acute diarrhea were studied for enteric pathogens. Patients positive for one or more pathogens were 359 (64.0%).

A simple immunoperoxidase method for detecting enteric adenovirus and rotavirus in cell culture.

A technique which includes the use of indirect immunoperoxidase antibody (IPA) has been developed for detecting enteric adenovirus and rotavirus antigens in cell cultures and has been compared with immunofluorescence antibody assay (IFA). The IPA technique was as sensitive as the IFA. The number of positive cells detected by both techniques in tissue cultures was the same; false positive results were not observed.

Evaluation of a new latex agglutination test for detecting human rotavirus in faeces.

Four methods for detecting rotaviruses (latex agglutination, electron microscopy, immunofluorescence and ELISA) have been compared on 57 faecal samples from children with acute diarrhoea. Complete agreement among the four techniques was found in 38 samples. One sample was positive by ELISA and latex agglutination but negative by the other two.

[Evaluation of the ELISA method of detection "anti-cytomegalovirus" antibodies].

The research of anti-Cytomegalovirus antibody by the ELISA (Enzyme-Linked-Immunosorbent-Assay) test has been compared with the complement fixation method. The results have shown that ELISA test was more sensitive than the complement fixation test and easy to carry out on a large scale.

[Detection of antibodies against rubella virus by means of radial hemolysis in gel].

Haemolysis-in-gel test for the detection of Rubella antibody has shown a good agreement with the results of haemoagglutination inhibition. This test was simple to perform, rapid, sensitive and could be well suited for screening of large numbers of serum samples.