Publications by authors named "Mazurova I"

A total of 78 patients (143 eyes) with low, medium and high degree myopia were assessed. Apart from standard ophthalmological tests, flowmetry and duplex ultrasound of ocular vessels were performed in all cases to evaluate volumetric and velocity parameters of ocular blood flow in relation to the length of anterior-posterior axis and the degree of myopia. It is found that both parameters of regional blood flow progressively decrease as the anterior-posterior axis increases.

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Aim: Characterization of contemporary C. diphtheriae strains isolated in Russia by using multilocus DNA sequencing (MLST).

Materials And Methods: 28 toxigenic C.

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Aim: Study structure ofa genetic determinant of amylase activity (amygene) in Corynebacterium diphtheriae biovar gravis and mitis strains.

Materials And Methods: 87 C. diphtheriae strains (31 gravis biovar strains and 56 mitis biovar strains) as well as C.

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The article deals with the results of studying the growth characteristics of nine nutrient mediums for primary plating of pathologic material. It is demonstrated that for successful and proper functioning of bacteriologic laboratory providing bacterial analysis for diphtheria the permanent quality control is needed to monitor the nutrient mediums for primary plating. The quality control is applied to evaluate the growth characteristics on such criteria as germination of isolated culture, intensity of its growth in 24 and 48 hours, optimal size of colonies characterized by their cultural characteristics, inhibiting activity concerning concurrent microflora.

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Aim: Genotyping of B. pertussis strains isolated from pertussis patients in Moscow.

Materials And Methods: 53 strains of B.

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Aim: Isolation and composition comparison of extracellular antigens (ECA) of pathogenic burkholderiae in SDS-PAGE electrophoresis and their use for differentiation of these microorganisms by immunodiffusion methods.

Materials And Methods: 60 Burkholderia pseudomallei strains, 14 B. mallei strains, 5 B.

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Aim: Evaluation of anti-pertussis antibodies in pertussis patients at different stages after the onset of the disease.

Materials And Methods: Levels of IgG, IgG1, IgG2, IgG3, IgG4, IgA and IgM antibodies against the antigen complex of pertussis were evaluated by enzyme immunoassay (EIA). Sera samples were analyzed from 208 pertussis patients examined from week 1 to 10 after the onset of the disease.

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Aim: To study activity of vaccine and circulating strains of Bordetella pertussis in serological reactions with serum samples from healthy vaccinated children and children with pertussis infection.

Materials And Methods: One hundred forty-six serum samples from children with pertussis infection as well as 158 samples from healthy vaccinated children aged 3 - 5 years old were studied. Serologic activity of 3 vaccine strains and 7 strains of B.

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Aim: To assess level of pertussin toxin (PT) production by vaccine strains of Bordetella pertussis and strains isolated from patients with whooping cough.

Materials And Methods: Concentration of PT in supernatants of microbial cultures of 3 vaccine strains and 25 strains of B. pertussis isolated from patients with pertussis in 2001 - 2005 was measured with enzyme immunoassay using gamma-globulin fractions of rabbit antiserum to PT as immunosorbent or included in peroxidase conjugates.

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In this article the results of practical use of central retinal zone (CRZ) tomography in primary open-angle glaucoma (POAG) measured with RTA (Retinal thickness Analyzer, Talia Technology Ltd., Israel) are presented. 460 patients (790 eyes) aged 45-85 years old were included into the study.

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The paper presents the results of practical application of a new screening method for determining tolerant intraocular pressure (TIOP) on a Blood Flow Analyzer (Paradigm, USA). Studies were conducted on 331 eyes (205 subjects). Group 1 consisted of 80 patients (126 eyes) without glaucoma being found, with intraocular pressure (IOP) in the average normal range, but with a TIOP excess of 1-8 mm Hg (a risk group); Group 2 included 15 subjects (20 eyes) without glaucoma with normal IOP in whom TIOP exceeded the existing IOP by 1-7 mm Hg; Group 3 comprised 110 patients (185 eyes) with Stage I-III primary open-angle glaucoma (POAG) with compensated IOP.

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Aim: Comparative analysis of structure of tcfA gene coding tracheal colonization factor of Bordetella pertussis strans isolated in Moscow from patients with pertussis.

Materials And Methods: Ninety-seven strains of B. pertussis isolated in different periods of pertussis infection epidemic process (1948 - 1989--from collection of Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology; 1990 - 2007--isolated in Moscow from patients with pertussis) were studied.

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The developed direct method for the laboratory diagnosis of pertussis, which is based on isothermal amplification technologies, has a high (100%) specificity and sensitivity (102 m.cl.), can detect the pathogen of the disease just in the clinical sample from a patient within 7-8 hours after start of the study.

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Aim: To study pathogenic characteristics of B. pertussis strains isolated from patients during different periods of pertussis infection epidemic process.

Materials And Methods: Strains of B.

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Aim: To assess antigenic composition consistency and serological characteristics of domestic acellular pertussis vaccine.

Materials And Methods: Amount of pertussis toxin, filamentous hemagglutinin, agglutinogens types 1, 2, and 3 in experimental batches of vaccine was measured by enzyme immunoassay. Levels of antibodies to aforementioned antigens as well as to lipopolysaccbaride in serum samples obtained from patients with pertussis and healthy vaccinated children were measured by the same method.

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The authors proposed a new screening for determining individual tolerant intraocular pressure (TIOP). The investigation covered 107 individuals (167 eyes). All the examinees were divided into three groups.

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Aim: To study clinical and laboratory data and levels of IgG, IgG1, IgG2, IgG3, IgG4, IgA and IgM to Bordetella pertussis complex of antigens in adults with prolonged cough.

Materials And Methods: Antibody levels to Bordetella pertussis complex of antigens were measured by solid phase immunoenzyme assay. Clinical and laboratory methods included CBC, chest X-ray, measurement of respiratory function, allergologic tests.

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Aim: To assess level of IgG1, IgG2, IgG3, and IgG4 to complex of antigens of Bordetella pertussis in patients with whooping cough and healthy children and adults.

Materials And Methods: Levels of anti-pertussis IgG subclasses in sera of patients with pertussis and healthy children and adults were measured with solid phase immunoenzyme assay using peroxidase-conjugated monoclonal antibodies to human IgG1, IgG2, IgG3, and IgG4.

Results: In patients with pertussis, IgG1-IgG3-IgG2-IgG4 type of distribution of subclasses with predominance of IgG1 and IgG3 was revealed.

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Polymorphism of tox and dtxR genes responsible for diphtheria toxin synthesis was revealed. Seven point mutations in tox gene were detected; study of their combinations allowed to determine 10 allelic variants of the tox gene in C. diphtheriae strains.

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Levels of IgG and IgA to complex of Bordetella pertussis antigens were assessed in 503 healthy children aged 1 - 14 years, 75 adolescents aged 15 - 17 years, and in 504 adults aged 18 - 54 years. The highest level of IgG was observed in children aged < 5 years. In older age groups progressive decrease of IgG level was noted, which more most prominent in 9 - 11 year-olds with subsequent stabilization of the level in adolescents and adults.

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Comparative study of IgG, IgA, and IgM levels to complex of antigens (CA) of vaccine strain No. 475 and separate antigens of Bordetella pertussis: pertussis toxin (PT), filamentous hemagglutinin (FHA), lypopolysaccharide (LPS), agglutinogens 1 (Aggl.1) and 2 (Aggl.

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Features of structure of different B. pertussis genes are studied in many countries of the world, and, first of all, ptxA gene, which encodes main protective antigen of the microbe--pertussis toxin. Starting from 1980s, B.

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Aim: Evaluation of the diagnostic value of pheno- and genotypic characteristics of B. cepacia strains collection.

Materials And Methods: Phenotypic and genetic methods of identification and differentiation of 25 strains of the B.

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The ocular drainage system in normal intraocular-pressure glaucoma was studied by fluorescence lymphoangioscopy of the anterior ocular segment on 24 eyes and by histomorphological analysis of the drainage zone on 12 eyes. The study revealed the increased volumetric lymph flow in the drainage zone, which suggests the higher tissue drainage that correlates with intraocular pressure and agrees with the histomorphological findings. Patients with normotensive glaucoma were found to have ischemic and destructive changes in the drainage zone and vascular system of the anterior ocular segment.

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Microbiological method of direct accelerated assessment of resistance of Mycobacterium tuberculosis to rifampicin and isoniazide was developed which is able to detect multidrug resistant M. tuberculosis 10-21 days after obtaining of sputum--4-5 times faster compared with the method of absolute concentrations. Efficacy of the method was 0.

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