A Novel Diagnostic and Treatment Approach to an Unusual Case of Dens Invaginatus in a Mandibular Lateral Incisor Using CBCT and 3D Printing Technology.

Background: This case report demonstrates the use of three-dimensional (3D) models produced from a cone beam computed tomographic (CBCT) volume to develop a treatment strategy for a rare type of dens invaginatus (DI) in a mandibular incisor.

Methods: A patient with DI Type IIIa presented for endodontic treatment. Following CBCT evaluation, the complex morphologic nature of the invagination required additional diagnostic tools for treatment planning.

A Potential Intracanal Medicament, 2-Hydroxyisocaproic Acid (HICA): Cytotoxicity, Genotoxicity, and Its Effect on SCAP Differentiation.

Intracanal medicaments with maximal antimicrobial efficacy and minimal damage to resident stem cells are essential for successful regenerative endodontic procedures. 2-Hydroxyisocaproic acid (HICA) could have the attributes of a potential intracanal medicament. This study evaluates its cytotoxicity, genotoxicity, and effects on the odontogenic and osteogenic differentiation of the stem cells of the apical papilla (SCAP).

Growth Factors Released from Advanced Platelet-Rich Fibrin in the Presence of Calcium-Based Silicate Materials and Their Impact on the Viability and Migration of Stem Cells of Apical Papilla.

Advanced platelet-rich fibrin (A-PRF) provides the scaffold and growth factors necessary for stem cells to proliferate and differentiate in successful regenerative endodontic procedures. This study investigates the release of transforming growth factor-β1 (TGF-β1), platelet-derived growth factor (PDGF), and vascular endothelial growth factor (VEGF) from A-PRF in cell culture media in the presence and absence of mineral trioxide aggregate (MTA) or Biodentine. Additionally, this research assesses the viability and migration of stem cells of the apical papilla (SCAP) in previously conditioned media.

Evaluation of Cannabinoids on the Odonto/Osteogenesis in Human Dental Pulp Cells In Vitro.

Introduction: Cannabinoids possess anti-inflammatory, analgesic, and osteogenic effects in different cell types and tissues. The null hypothesis is delta-9-tetrahydrocannabinol (THC) might induce dental tissue repair and regeneration. The aim of this study was to investigate the effect of THC on human dental pulp cell (HDPC) viability and biomineralization as well as the molecular mechanism of THC-induced odonto/osteogenic differentiation of HDPCs.

Volumetric Evaluation of 5 Root Canal Obturation Methods in TrueTooth 3-dimensional-Printed Tooth Replicas Using Nano-computed Tomography.

Introduction: The aim of this study was to evaluate the volumes of total obturation and voids in different obturation techniques using nano-computed tomographic imaging. The null hypothesis was that the obturation technique and the materials used have no effect on the total volume of obturation or the total volume of voids.

Methods: Fifty maxillary left central incisor 3-dimensional-printed replicas (TrueTooth; Dental Engineering Laboratories, Santa Barbara, CA) were instrumented and randomly assigned to 5 different obturation groups (n = 10): single cone with EndoSequence Gutta-Percha Points (Brasseler USA, Savannah, GA) and Ribbon Sealer (Dentsply Sirona, Tulsa, OK) (SC1), single cone with BC 150 Series Gutta-Percha Points (Brasseler USA) and EndoSequence BC Sealer (Brasseler USA) (SC2), continuous wave with EndoSequence Gutta-Percha Points and Ribbon Sealer (CW), GuttaCore carrier obturation (Dentsply Sirona) and Ribbon Sealer (GC), and cold lateral condensation with EndoSequence Gutta-Percha Points and Ribbon Sealer (CL).

Micro-computed Tomographic Evaluation of the Shaping Ability of WaveOne Gold, TRUShape, EdgeCoil, and XP-3D Shaper Endodontic Files in Single, Oval-shaped Canals: An In Vitro Study.

Introduction: This study evaluated and compared the shaping ability of the WaveOne Gold (Dentsply/Tulsa Dental Specialties, Tulsa, OK), TRUShape 3D Conforming File (Dentsply/Tulsa Dental Specialties), EdgeCoil (EdgeEndo, Albuquerque, NM), and XP-3D Shaper (Brasseler USA, Savannah, GA) endodontic file systems on oval-shaped canals using micro-computed tomographic (micro-CT) technology.

Methods: Thirty-two oval-shaped, single-canal extracted human teeth were decoronated 1 mm coronal to the cementoenamel junction and scanned via a micro-CT scanner (μCT100; Scanco Medical, Bassersdorf, Switzerland). Teeth were divided into 4 groups (n = 8) and instrumented according to the manufacturer's instructions.

Cytotoxicity and Genotoxicity of a New Intracanal Medicament, 2-hydroxyisocaproic Acid-An In Vitro Study.

Introduction: A successful outcome of root canal therapy relies on effective disinfection of the root canal system, including the use of intracanal medicaments, which vary in their bactericidal and cytotoxic properties. Assessing the benefits and risks associated with the use of these medicaments is of extreme importance, especially in regenerative endodontic procedures, because residual stem cells may be harmed. In this study, we tested the cytotoxicity and genotoxicity of a novel agent, 2-hydroxyisocaproic acid (HICA), and compared its properties with those of a well-established medicament, calcium hydroxide.

The Effect of Temperature on Cyclic Fatigue of Nickel-titanium Rotary Endodontic Instruments.

Introduction: This study examined the effect of different temperatures on the cyclic fatigue of nickel-titanium rotary files.

Methods: Three groups of nickel-titanium rotary files (EF group [EdgeFile; EdgeEndo, Albuquerque, NM], VB group [Vortex Blue; Dentsply Tulsa Dental Specialties, Tulsa, OK], and ESX group [ESX; Brasseler USA, Savannah, GA]) of size 25 with a .04 taper and 25-mm length were tested in a metal block that simulated a canal curvature of 60° and a 5-mm radius curvature.