Characterization of Cell Surface by the Microbial Adhesion to Solvents Method.

The cell surface physicochemical properties of should influencing the dispersal and adsorption of spores and hyphae in soil and should conditioning there interactions with organic or metal substances in the bioremediation of contaminated environment. These properties are concerning surface hydrophobicity, electron donor/acceptor, and charge surface. To date, only hydrophobicity of was studied by contact angle measurements and microbial adhesion to hydrocarbons (MATH).

Failure of beta-lactam antibiotics and marked efficacy of fluoroquinolones in treatment of murine Yersinia pseudotuberculosis infection.

The treatment of yersiniosis by beta-lactams is questionable considering the proven failure of newer beta-lactams for treating murine Yersinia enterocolitica infection. Another modality of experimental treatment was performed with a virulent strain of Y. pseudotuberculosis (nonproducer of beta-lactamase) highly susceptible (in terms of MICs) to amoxicillin, cefotaxime, ceftriaxone, imipenem, doxycycline, gentamicin, and ofloxacin.

BamHI restriction endonuclease analysis of Yersinia ruckeri plasmids and their relatedness to the genus Yersinia 42- to 47-megadalton plasmid.

The plasmid profile and BamHI restriction pattern of 17 sorbitol-negative and 1 sorbitol-positive French Yersinia ruckeri strain of the American type strain were studied. The 17 sorbitol-negative strains and the American strain harbored a 62-megadalton (MDa) plasmid with an identical BamHI restriction pattern. Southern hybridization indicated that this 62-MDa plasmid is common among these various strains.

Expression of iron-regulated proteins in Yersinia species and their relation to virulence.

Under iron-starvation conditions, the different Yersinia species expressed various iron-regulated proteins. Among them, two high-molecular-weight outer membrane proteins were synthesized in high-virulence-phenotype Y. pestis, Y.

Infrequent detection of Yersinia enterocolitica in childhood diarrhea in Bangladesh.

In a search for Yersinia infection in Bangladesh, one isolate of Y. enterocolitica serotype 0:3 was obtained from stools of 1,450 children with fever and diarrhea and one of Y. enterocolitica serotype 0:8 was recovered from intestinal contents of 80 fatal diarrheal cases during postmortem examination.

Protection against Yersinia infection induced by non-virulence-plasmid-encoded antigens.

Specific immunity against Yersinia was induced by plasmid-encoded antigens not associated with virulence. Mice were immunised with viable bacteria from a virulence-plasmid-cured strain of Y. pseudotuberculosis.

[Double infection with Yersinia enterocolitica 0:3 and 0:9].

An unusual case of Yersiniosis was observed in a 20-year-old woman. Except for an extraordinarily severe onset the clinical course was not remarkable. But two different strains of Yersinia were repeatedly isolated.

Lack of correlation between plasmid-encoded outer membrane proteins and virulence of Yersinia enterocolitica.

When various strains of Yersinia enterocolitica belonging to serovars 0:1,3, 0:3, 0:5,27, 0:9 and 0:Tacoma harbouring 44- to 47-Md plasmids, or their spontaneously cured isogenic pairs, were inoculated (i. v., with standardized inocula) into Swiss female mice, the kinetics of bacterial survival in the spleen were followed, revealing inoculum destruction within 15 days.

Growth of Yersinia pseudotuberculosis in mouse spleen despite loss of a virulence plasmid of mol. wt 47 X 10(6).

A highly virulent strain of Yersinia pseudotuberculosis (LD50 c. 10(2) bacteria/mouse) harboured two plasmids with mol. wt of 47 X 10(6) and 61 X 10(6).

Role of the virulence-associated plasmids of Yersinia enterocolitica on its immunogenicity against Y. pestis.

Forty-two strains selected from various serovars of Yersinia enterocolitica were studied for their ability to induce cross-protection against Y. pestis. Only primo-infected mice (i.

Simple method for demonstration of differential colony morphology of plasmid-associated virulent clones of Yersinia enterocolitica.

Virulent (plasmid-associated) strains of Yersinia enterocolitica grown on RPMI 1640 agar (RPMI-1640 medium [Flow Laboratories] with 40 mM HEPES and 1.5% [wt/vol] purified agar [Difco]) dissociated into small and large colonies. The autoagglutination test (a marker of virulence) is regularly positive with small colonies and negative with large colonies.

Temperature-modulated immunogenicity to Yersinia pestis from Yersinia enterocolitica O3.

The ability of Yersinia enterocolitica O3, grown at 25 degrees C, to promote cross-immunity to Y. pestis was lost after repeated subcultures at 37 degrees C, which selected for bacterial populations having lower in vivo survival. Subculturing Y.

Cellular depletion in Peyer's patches after cyclophosphamide treatment in mice.

The cytotoxic effects of cyclophosphamide (CY) in mice given by the intravenous route at the dose of 200 mg per kg of body weight, were investigated in Peyer's patches comparatively with peripheral blood and mesenteric lymph node as well as changes in the spleen weight. Peyer's patches are the sites of a more intense and durable cellular depletion than the mesenteric lymph node, observed as soon as day 1. A partial restauration of cell populations was observed from the 9th day, but even at day 21 their number remained lower than in control mice.

[Experimental infection in mice with Yersinia enterocolitica (strain biotype 4, serogroup O:3, phage type VIII): growth of the inoculum in athymic or cyclophosphamide treated mice (author's transl)].

Intravenous infection of Swiss mice with a strain of Yersinia enterocolitica unable to colonize normal mice by the oral route, induced a systemic infection. Viable bacteria were isolated from homogenates of liver, spleen and lungs, as early as one hour after the challenge and have been detected during two weeks. On the other hand, the number of viable bacteria isolated from the blood has always been very low.