The Content of Anthocyanins in Cowpea ( (L.) Walp.) Seeds and Contribution of the Gene Cluster to Their Coloration Pattern.

The intensively pigmented legumes belonging to and spp. are valued as an essential component of healthy nutrition due to their high content of flavonoids. In this context, we used the accessions of with different colors of seed coats from the N.

Peschkova from Yakutia: Extraction and Mass Spectrometric Characterization of 128 Chemical Compounds.

Peschkova is a rare and endangered species of the genus of the Lamiaceae family. The species was first described in 1997 and listed in the Red Data Book of Yakutia. Significant differences in the multicomponent composition of extracts from collected in the natural environment and successfully introduced in the Botanical Garden of Yakutsk were identified by a team of authors earlier in a large study.

Rupr. et Maxim.: Supercritical CO Extraction and Mass Spectrometric Characterization of Chemical Constituents.

Three types of extraction were used to obtain biologically active substances from the heartwood of : supercritical CO extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yield of biologically active substances. Several experimental conditions were investigated in the pressure range of 50-400 bar, with 2% of ethanol as co-solvent in the liquid phase at a temperature in the range of 31-70 °C.

Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach.

In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon.

Identification and Spatial Distribution of Bioactive Compounds in Seeds (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry.

The research presents a comparative metabolomic study of extracts of seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources.

Spatial Distribution of Polyphenolic Compounds in Corn Grains ( L. var. ) Studied by Laser Confocal Microscopy and High-Resolution Mass Spectrometry.

Desirable changes in the biochemical composition of food plants is a key outcome of breeding strategies. The subsequent localization of nutritional phytochemicals in plant tissues gives important information regarding the extent of their synthesis across a tissue. We performed a detailed metabolomic analysis of phytochemical substances of grains from L.

Phytochemical Analysis of Phenolics, Sterols, and Terpenes in Colored Wheat Grains by Liquid Chromatography with Tandem Mass Spectrometry.

The colored grain of wheat ( L.) contains a large number of polyphenolic compounds that are biologically active ingredients. The purpose of this work was a comparative metabolomic study of extracts from anthocyaninless (control), blue, and deep purple (referred to here as black) grains of seven genetically related wheat lines developed for the grain anthocyanin pigmentation trait.

Simultaneous Determination of 78 Compounds of Extract by Supercritical CO-Extraction and HPLC-ESI-MS/MS Spectrometry.

The plant L. of family was extracted using the supercritical CO-extraction method. Several experimental conditions were investigated in the pressure range of 200-500 bar, with the used volume of cosolvent ethanol in the amount of 1% in the liquid phase at a temperature in the range of 31-70°C.

LC-MS/MS Screening of Phenolic Compounds in Wild and Cultivated Grapes Rupr.

This work represents a comparative metabolomic study of extracts of wild grapes obtained from six different places in the Primorsky and Khabarovsk territories (Far East Russia) and extracts of grapes obtained from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources (St.

The potential application of supercritical CO in microbial inactivation of food raw materials and products.

The purpose of this study was to review the possibility of using supercritical CO as a green and sustainable technology for microbial inactivation of raw material for further application in the food industry. The history of the development of supercritical CO microbial inactivation has been widely described in this article. The fundamental scientific part of the process like mechanism of bactericidal action of CO or inactivation of key enzymes were characterized in detail.

Features and Advantages of Supercritical CO Extraction of Sea Cucumber Semper, 1868.

Extraction process of Semper, 1868, which are subspecies of (Gunnerus, 1767), were studied. It was shown that supercritical carbon dioxide extraction of holothuria was more effective than conventional solvent extraction. Step-by-step extraction with carbon dioxide followed by supercritical extraction with the addition of a co-solvent of ethanol can almost double the yields of extracts of triterpene glycosides, styrenes and carotenoids.

Comparative Analysis of Far East Sikhotinsky Rhododendron () and East Siberian Rhododendron () Using Supercritical CO-Extraction and HPLC-ESI-MS/MS Spectrometry.

Pojark. and Rheder have been actively used in ethnomedicine in Mongolia, China and Buryatia (Russia) for centuries, as an antioxidant, immunomodulating, anti-inflammatory, vitality-restoring agent. These plants contain various phenolic compounds and fatty acids with valuable biological activity.

Telomerase and telomeres in aging theory and chronographic aging theory (Review).

The current review focuses on the connection of telomerase and telomeres with aging. In this review, we describe the changes in telomerase and telomere length (TEL) during development, their role in carcinogenesis processes, and the consequences of reduced telomerase activity. More specifically, the connection of TEL in peripheral blood cells with the development of aging‑associated diseases is discussed.

Rapid Mass Spectrometric Study of a Supercritical CO-extract from Woody Liana by HPLC-SPD-ESI-MS/MS.

Woody liana contains valuable lignans, which are phenylpropanoids with valuable biological activity. Among green and selective extraction methods, supercritical carbon dioxide (SC-CO) was shown to be the method of choice for the recovery of these naturally occurring compounds. Carbon dioxide (CO) was the solvent with the flow rate (10-25 g/min) with 2% ethanol as co-solvent.

Supercritical CO Extraction and Identification of Ginsenosides in Russian and North Korean Ginseng by HPLC with Tandem Mass Spectrometry.

Ginseng roots, C.A. Meyer, obtained from cultivated ginseng grown in the Kaesong province (North Korea) and Primorye (Russia) were extracted using the supercritical CO extraction method.

Discovery of potent telomerase activators: Unfolding new therapeutic and anti-aging perspectives.

Telomere length, a marker of cellular aging, decreases with age and it has been associated with aging‑related diseases. Environmental factors, including diet and lifestyle factors, affect the rate of telomere shortening which can be reversed by telomerase. Telomerase activation by natural molecules has been suggested to be an anti‑aging modulator that can play a role in the treatment of aging‑related diseases.

Panax ginseng components and the pathogenesis of Alzheimer's disease (Review).

Ginseng is one of the main representatives of traditional Chinese medicine and presents a wide range of pharmacological actions. Ginsenosides are the main class of active compounds found in ginseng. They demonstrate unique biological activity and medicinal value, namely anti-tumour, anti‑inflammatory and antioxidant properties, as well as anti-apoptotic properties.