Intra-laboratory validation of yeast-based reporter gene assays for human thyroid hormone receptors.

Thyroid hormones (THs) function by activating TH receptors (THRα and THRβ) on the target cells. Several chemicals adversely affect human health and ecosystems by disrupting TH signaling. Multiple assays for thyroid disruption have been reviewed for validation, especially with respect to assay reliability, sensitivity, efficiency, and technical criteria in the OECD Test Guidelines framework.