Optimization of canine interleukin-12 production using a baculovirus insect cell expression system.

Background: Interleukin-12 is an important cytokine in mediating cellular immune responses.

Results: Recombinant single-chain canine IL-12 was produced in a baculovirus-insect cell system with the aim of conducting further studies on modulation of immune responses in dogs. To optimize the production of recombinant canine IL-12, a classical baculovirus and a modified vector (chitinase A and v-cathepsin knockout) were used containing a native or an optimized insert of canine IL-12.