RNAi-mediated knockdown of inhibits tumorigenicity of human glioblastoma cells.

In a previous genome-wide expression profiling study, we identified as a hyperexpressed gene in stem-like cells of distinct glioblastoma multiforme (GBM) specimens. Since the encoded E2F2 transcription factor has been implicated in both tumor suppression and tumor development, we conducted a functional study to investigate the pertinence of to human gliomagenesis. expression was knocked down by transfecting U87MG cells with plasmids carrying a specific silencing shRNA.