Potential secondary metabolite from Indonesian Actinobacteria (InaCC A758) against .

Objectives: This study explored Indonesian Actinobacteria which were isolated from endophytic microbes and mangrove ecosystem for new antimycobacterial compounds.

Materials And Methods: Antimycobacterial activity test was carried out against H37Rv. Chemical profiling of secondary metabolite using Gas Chromatography-Mass Spectroscopy (GC-MS) and High Resolution-Mass Spectroscopy (HR-MS) was done to the ethyl acetate extract of active strain InaCC A758.

Evaluation of crystal violet decolorization assay and resazurin microplate assay for antimycobacterial screening.

The main obstacle in antimycobacterial discovery is the extremely slow growth rates of pathogenic mycobacteria that lead to the long incubation times needed in antimycobacterial screening. Some testings has been developed and are currently available for antimycobacterial screening. The aim of the study was to compare Resazurin Microplate Assay (REMA) and Crystal Violet Decolorization Assay (CVDA) for testing mycobacteria susceptibility to isoniazid and rifampicin as well as for antimycobacterial screening of natural products (NP).