Publications by authors named "Maximilian Mantel"
Article Synopsis
- Protein labeling using DNA oligonucleotides with attached fluorophores is popular in advanced microscopy for imaging and quantifying multiple targets.
- A significant issue with this method is the high background noise from unbound fluorophores, which can interfere with results.
- This study introduces a technique where fluorophore dimers self-quench to lower background noise, enhancing imaging quality in super-resolution microscopy techniques like stimulated emission depletion and single-molecule localization microscopy.
Self-quenched Fluorophore Dimers for DNA-PAINT and STED Microscopy.
Angew Chem Int Ed Engl
September 2023
Super-resolution techniques like single-molecule localisation microscopy (SMLM) and stimulated emission depletion (STED) microscopy have been extended by the use of non-covalent, weak affinity-based transient labelling systems. DNA-based hybrid systems are a prominent example among these transient labelling systems, offering excellent opportunities for multi-target fluorescence imaging. However, these techniques suffer from higher background relative to covalently bound fluorophores, originating from unbound fluorophore-labelled single-stranded oligonucleotides.
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