Multi-scale phenotyping of senescence-related changes in roots of Rapeseed in response to nitrate limitation.

Root senescence remains largely unexplored. In this study, the temporality of the morphological, metabolic, and proteomic changes occurring with root aging were investigated, providing a comprehensive picture of the root senescence program. We found novel senescence-related markers for the characterization of the developmental stage of root tissues.

A New Role for SAG12 Cysteine Protease in Roots of .

Senescence associated gene (SAG) 12, which encodes a cysteine protease is considered to be important in nitrogen (N) allocation to seeds. A decrease in the yield and N content of the seeds was observed in the Arabidopsis SAG12 knockout mutants () relative to the wild type (Col0) under limited nitrogen nutrition. However, leaf senescence was similar in both lines.

SAG12, a Major Cysteine Protease Involved in Nitrogen Allocation during Senescence for Seed Production in Arabidopsis thaliana.

SAG12 is the most widely used senescence-associated reference gene for characterizing leaf senescence, and the increase in SAG12 protein during leaf senescence is remarkable. However, the role of this cysteine protease in N remobilization and the leaf senescence process remains unclear. The role of SAG12 has been poorly investigated and the few reports dealing with this are somewhat controversial.