Chemical Annealing Restructures RNA for Nanopore Detection.

RNA is a key biochemical marker, yet its chemical instability and complex secondary structure hamper its integration into DNA nanotechnology-based sensing platforms. Relying on the denaturation of the native RNA structure using urea, we show that restructured DNA/RNA hybrids can readily be prepared at room temperature. Using solid-state nanopore sensing, we demonstrate that the structures of our DNA/RNA hybrids conform to the design at the single-molecule level.

Emerging Approaches to DNA Data Storage: Challenges and Prospects.

With the total amount of worldwide data skyrocketing, the global data storage demand is predicted to grow to 1.75 × 10 GB by 2025. Traditional storage methods have difficulties keeping pace given that current storage media have a maximum density of 10 GB/mm.

Observing capture with a colloidal model membrane channel.

We use video microscopy to study the full capture process for colloidal particles transported through microfluidic channels by a pressure-driven flow. In particular, we obtain trajectories for particles as they move from the bulk into confinement, using these to map in detail the spatial velocity and concentration fields for a range of different flow velocities. Importantly, by changing the height profiles of our microfluidic devices, we consider systems for which flow profiles in the channel are the same, but flow fields in the reservoir differ with respect to the quasi-2D monolayer of particles.