Multimodal, PSMA-Targeted, PAMAM Dendrimer-Drug Conjugates for Treatment of Prostate Cancer: Preclinical Evaluation.

Introduction: Prostate cancer (PC) is the second most common cancer and the fifth most frequent cause of cancer death among men. Prostate-specific membrane antigen (PSMA) expression is associated with aggressive PC, with expression in over 90% of patients with metastatic disease. Those characteristics have led to its use for PC diagnosis and therapies with radiopharmaceuticals, antibody-drug conjugates, and nanoparticles.

Antimicrobial activity of Lactiplantibacillus plantarum against shiga toxin-producing Escherichia coli.

Aims: The aim of the present work was to characterize the Lactiplantibacillus sp. LP5 strain, isolated from pork production, and identify bacteriocin-like inhibitory substances produced by this strain.

Methods And Results: In this study, LP5 was identified by species-specific PCR and 16S rRNA sequencing.

Molecular and Genetic Characterization of Colicinogenic Strains Active against Shiga Toxin-Producing O157:H7.

The objective of this work was to molecularly and genotypically characterize and test the inhibitory activity of six colicinogenic strains (ColEc) and their partially purified colicins against STEC O157:H7 isolated from clinical human cases. Inhibition tests demonstrated the activity of these strains and their colicins against STEC O157:H7. By PCR it was possible to detect colicins Ia, E7, and B and microcins M, H47, C7, and J25.

The latent reservoir of inducible, infectious HIV-1 does not decrease despite decades of antiretroviral therapy.

HIV-1 persists in a latent reservoir in resting CD4+ T cells despite antiretroviral therapy (ART). The reservoir decays slowly over the first 7 years of ART (t1/2 = 44 months). However, whether decay continues with long-term ART is unclear.

Safety and Immunogenicity of a Chimeric Subunit Vaccine against Shiga Toxin-Producing in Pregnant Cows.

Shiga toxin-producing (STEC) is a zoonotic pathogen that causes gastroenteritis and Hemolytic Uremic Syndrome. Cattle are the main animal reservoir, excreting the bacteria in their feces and contaminating the environment. In addition, meat can be contaminated by releasing the intestinal content during slaughtering.

Carbapenem-resistant bloodstream infections: A case-control study from a pediatric referral hospital in Argentina.

Background: Antibiotic-resistant gram-negative bloodstream infections (BSI) remain a leading cause morbidity and mortality in pediatric patients with a high impact on the public health system. Data in resource-limited countries, including those in Latin America and the Caribbean region, are scarce. The aim of the study was to identify risk factors for acquiring carbapenem-resistant (CRE) bacteremia in children and to assess the use of resources.

Experience with ultrasound use in central venous catheterization (jugular-femoral) in pediatric patients in an intensive care unit.

Objective: Describe ultrasound-guided central venous catheterization use comparing the number of attempts (1 versus 2 or more attempts) in relation to catheters placed in the internal jugular vein (IJV) versus the femoral vein (FV).

Material And Methods: Descriptive, prospective study of central venous catheters (CVCs) inserted via ultrasound-guided puncture in patients aged 1 month to 18 years. A multivariate regression model was done considering the primary endpoint, first puncture success in relation to the insertion site (IJV versus FV), and predictors of success.

Improvement in communication during patient handoff between areas from a children's hospital.

Introduction: Patient handoff is an interactive process including data communication and responsible transfer in order to safely maintain the continuity of care. Failure in this process may result in inadequate care and favor the occurrence of errors.

Objective: To implement a standardized instrument for patient handoff from the intensive care unit (ICU) to the intermediate-medium care unit (IMCU), and compare communication between health care providers before and after the intervention.

Chiral Recognition of Dansyl Derivatives with an Amino Acid-Based Molecular Micelle: A Molecular Dynamics Investigation.

In this study, the chiral separation mechanisms of Dansyl amino acids, including Dansyl-Leucine (Dans-Leu), Dansyl-Norleucine (Dans-Nor), Dansyl-Tryptophan (Dans-Trp) and Dansyl-Phenylalanine (Dans-Phe) binding to poly-sodium -undecanoyl-(L)-Leucylvalinate, poly(SULV), were investigated using molecular dynamics simulations. Micellar electrokinetic chromatography (MEKC) has previously shown that when separating the enantiomers of these aforementioned Dansyl amino acids, the L- enantiomers bind stronger to poly(SULV) than the D- enantiomers. This study aims to investigate the molecular interactions that govern chiral recognition in these systems using computational methods.

Evaluation of food baits to capture Drosophila suzukii in the southern of Brazil.

Drosophila suzukii is one of the main pests of small fruits in the world. An effective monitoring is fundamental to detect the presence of the fly and to predict the infestation of fruits in new areas. We evaluated the food baits Ceratrap®, Torula®, Biofruit®, Suzukii Trap®, apple cider vinegar, and a homemade mixture comprising wine, vinegar and molasses (WVM) for D.

[Prognostic factors predicting failure of the trial without catheter after tamsulosin in patients with acute urinary retention.].

Objective: To analyze prognostic variables that impact on the trial without catheter after tamsulosin in patients with acute urine retention.

Methods: Retrospective analysis of a prospective database of a cohort of successive patients with acute urinary retention due to benign prostatic hyperplasia attended at the urology emergency room during one year time period. We analyzed the clinical and radiological characteristics and the international prostatic symptom score questionnaire of a total 65 male patients.

Biological characteristics of Grapholita molesta (Lepidoptera: Tortricidae) induced to diapause in laboratory.

In southern Brazil, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) uses diapause as survival strategy during the winter (May-August). In our study, we evaluated the effect of diapause on biological characteristics of the species for 4 months in laboratory. Newly hatched larvae of G.

Dam methylation regulates the expression of SPI-5-encoded sopB gene in Salmonella enterica serovar Typhimurium.

DNA adenine methylation is an essential factor in Salmonella virulence. Here, we investigate the involvement of DNA adenine methylase (Dam) in the expression and translocation of a SPI-5-encoded effector of S. Typhimurium.

Control of Grapholita molesta (Busck, 1916) (Lepidoptera: Tortricidae) with entomopathogenic nematodes (Rhabditida: Heterorhabditidae, Steinernematidae) in peach orchards.

Oriental fruit moth Grapholita molesta (Busck, 1916) (Lepidoptera: Tortricidae) is considered a major pest in temperate fruit trees, such as peach and apple. Entomopathogenic nematodes (EPNs) are regarded as viable for pest management control due to their efficiency against tortricid in these trees. The objective of this study was to evaluate the effectiveness of native EPNs from Rio Grande do Sul state against pre-pupae of G.

Activity of eight strains of entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) against five stored product pests.

Stored product pests are responsible for losses that can amount 10% during cereal storage in the world. Aiming to find an alternative method to the chemicals used for the stored-product pests, eight strains of entomopathogenic nematodes (EPNs) were tested against five species of stored product pests. The bioassays were conducted in microtubes containing paper, inoculated with EPNs and insect diet.

Evaluation of efficacy of 18 strains of entomopathogenic nematodes (Rhabditida) against Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) under laboratory conditions.

Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) is an important plant virus vector in grapevine crops in Brazil and other countries. The mealybug grows in roots and leaves of the grapes. Entomopathogenic nematodes (EPNs) are efficient control agents against insects associated to the soil and could be applied with the same equipment used for chemical insecticides.

Biology and fertility life table of the green aphid Chaetosiphon fragaefolli on strawberry cultivars.

Our objective was to study the biology and develop a fertility life table for the aphid Chaetosiphon fragaefolli (Cockerell) (Hemiptera: Aphididae) on leaves of strawberry, Fragaria × ananassa, Duchesne ex Rozier (Rosales: Rosaceae), of the cultivars Albion, Aromas, Camarosa, Camino Real, Diamante, Earlibrite, and Saborosa. This study was conducted under controlled conditions: 25 ± 1 (°)C, 70 ± 10% RH, and 12:12 L:D . Arenas were set up consisting of leaves inside Petri dishes containing 3% agar.

Effects of azadirachtin on Tetranychus urticae (Acari: Tetranychidae) and its compatibility with predatory mites (Acari: Phytoseiidae) on strawberry.

Background: The spider mite, Tetranychus urticae, is the major strawberry pest in Brazil. The main strategies for its control comprise synthetic acaricides and predatory mites. The recent register of a commercial formula of azadirachtin (Azamax(®) 12 g L(-1) ) can be viable for control of T.

[Non invasive mechanical ventilation in Pediatric Intensive Care, four years of clinical practice].

Unlabelled: In the last years, different studies have shown the effectiveness of noninvasive ventilation (NIV) in reducing rate of intubation in chronic and acute respiratory failure, with the direct consequence of lower morbidity and mortality associated with artificial airway, and reduction of days of hospitalization in ICU.

Objective: To describe our clinical experience in the use of NIV in the pediatric intensive care unit (PICU) and to analyze the characteristics associated with the success of this technique.

Design: Retrospective, descriptive and observational study.

[Occurrence of Ctenarytaina eucalypti (Maskell) (Hemiptera: Psyllidae) and its natural enemy Psyllaephagus pilosus Noyes (Hymenoptera: Encyrtidae) in Eucalyptus globulus in the state of Rio Grande do Sul, Brazil].

Eucalyptus are hosts of several psyllid species. Among them, Ctenarytaina eucalypti (Maskell) is considered the most harmful to Eucalyptus globulus plantations. In Brazil, this pest has been observed in the state of Parana, and in 2005, it was found causing damage to plantations of E.

Influenza A (pH1N1) infection in children admitted to a pediatric intensive care unit: differences with other respiratory viruses.

Objectives: To describe the clinical features of patients admitted to the pediatric intensive care unit (PICU) with acute lower respiratory tract infection (LRTI) attributable to influenza A pH1N1 virus and compare them with those admitted with LRTI due to other viral pathogens.

Design: Retrospective, observational, comparative study.

Setting: PICU in a university-affiliated, tertiary-care, pediatric hospital.

Efficacy of indigenous entomopathogenic nematodes (Rhabditida: Heterorhabditidae, Steinernematidae), from Rio Grande do Sul Brazil, against Anastrephafraterculus (Wied.) (Diptera: Tephritidae) in peach orchards.

Laboratory, greenhouse, and field experiments were performed with the objective of selecting efficient indigenous strains of entomopathogenic nematodes (EPNs) from Rio Grande do Sul (RS) state, Brazil, for controlling the South American fruit fly, Anastrepha fraterculus (Wied.). Laboratory experiments were conducted in 24 well-plates filled with sterile sand and one insect per well.

[Occurrence of Euetheola humilis (Burmeister) (Coleoptera: Scarabaeidae) in Eucalyptus saligna Smith (Myrtaceae), in Rio Grande do Sul State, Brazil].

Euetheola humilis (Burmeister) is a scarabaeid pest of rice, sugarcane and corn cultures, that attacks the crops during both larval and adult phase. The occurrence of E. humilis was observed in a forest area with Eucalyptus saligna Smith, from Votorantim Celulose & Papel (Fazenda Aroeira), in the Municipality of Candiota, Rio Grande do Sul State.