Publications by authors named "Maurizio Tiarca"
Article Synopsis
- Traditional analysis of microbial communities mainly uses PCR amplification of 16S rRNA genes, which can introduce biases and miss undiscovered species due to varying primer affinities.
- DNA-based methods often fail to reflect the actual physiological roles of different microbes in an environment and can be influenced by the variable number of rRNA operons in genomes.
- A new method of direct sequencing 16S rRNA without PCR was tested in an anammox bioreactor, showing consistent results that align with biochemical processes, suggesting this approach should complement existing PCR-based methods rather than replace them.
