Long-Term Conservation for the Safeguard of : An Endemic and Endangered Species of Sicily.

The combined approaches between ex situ and in situ conservation are of great importance for threatened species in urgent need of protection. This study aims to develop concrete actions to preserve the relic of 30 adult trees of the Sicilian fir () from extinction using long-term germplasm conservation in liquid nitrogen (LN, -196 °C). Pollen grains were collected, and their moisture content (MC) was measured.

Plant Cryopreservation: Principles, Applications, and Challenges of Banking Plant Diversity at Ultralow Temperatures.

Progressive loss of plant diversity requires the protection of wild and agri-/horticultural species. For species whose seeds are extremely short-lived, or rarely or never produce seeds, or whose genetic makeup must be preserved, cryopreservation offers the only possibility for long-term conservation. At temperatures below freezing, most vegetative plant tissues suffer severe damage from ice crystal formation and require protection.

Efficient Protocol for Improving the Development of Cryopreserved Embryonic Axes of Chestnut ( Mill.) by Encapsulation-Vitrification.

An optimized cryopreservation protocol for embryonic axes (EAs) of chestnut ( Mill.) has been developed based on the encapsulation-vitrification procedure. EAs of mature seeds were aseptically dissected and encapsulated in alginate beads with or without 0.

Large-Scale Plant Production of L. by Liquid Culture in Temporary Immersion System and Possible Application to the Synthesis of Bioactive Substance.

Goji ( L.) has recognized nutritive and antioxidant properties and many products are commercialized for health in food market. Besides its food use, goji has been the subject of more than 2000 years of traditional Chinese medicine, using berries, root bark, and leaves.

Impact of two arbuscular mycorrhizal fungi on Arundo donax L. response to salt stress.

AM symbiosis did not strongly affect Arundo donax performances under salt stress, although differences in the plants inoculated with two different fungi were recorded. The mechanisms at the basis of the improved tolerance to abiotic stresses by arbuscular mycorrhizal (AM) fungi have been investigated mainly focusing on food crops. In this work, the potential impact of AM symbiosis on the performance of a bioenergy crop, Arundo donax, under saline conditions was considered.

Cryotechniques for the Long-Term Conservation of Embryogenic Cultures from Woody Plants.

Since its development in the 1960s, plant cryopreservation is considered an extraordinary method of safe long-term conservation of biological material, as it does not induce genetic alterations and preserve the regeneration potential of the stored material. It is based on the storage of explants at cryogenic temperatures, such as the one of liquid nitrogen (-196 °C), where the metabolism within the cells is suspended; thus, the time for these cells is theoretically "stopped". Cryopreservation is particularly important for embryogenic cultures, as they require periodic subculturing for their maintenance, and this, in turn, increases the risk of losing the material, as well as its embryogenic potential.

In vitro propagation of peanut (Arachis hypogaea L.) by shoot tip culture.

Peanut (Arachis hypogaea L.), also known as groundnut, is the most important species of Arachis genus, originating from Brazil and Peru. Peanut seeds contain high seed oil, proteins, amino acids, and vitamin E, and are consumed worldwide as edible nut, peanut butter, or candy, and peanut oil extracted from the seeds.

In vitro propagation of olive (Olea europaea L.) by nodal segmentation of elongated shoots.

Olive (Olea europaea L.), long-living, ever-green fruit tree of the Old World, has been part of a traditional landscape in the Mediterranean area for centuries. Both the fruits consumed after processing and the oil extracted from the fruits are among the main components of the Mediterranean diet, widely used for salads and cooking, as well as for preserving other food.

Effects of osmotic pretreatments on oxidative stress, antioxidant profiles and cryopreservation of olive somatic embryos.

A three-day pretreatment of olive somatic embryos (SE) with 0.75 M sucrose, combined with cryoprotection (0.5M DMSO, 1M sucrose, 0.

Cryogenic technologies for the long-term storage of Citrus germplasm.

With its beautiful trees, Citrus species have long been valued by humanity. The tasteful fruits, extensively used for nutrition, are also good for health due to the high content in vitamins, minerals, and dietary fibers. Like majority of the woody fruit plants, Citrus germplasm is conserved mainly as field collections in clonal orchards.

In vitro conservation and cryopreservation of ornamental plants.

Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage) or in liquid nitrogen (cryopreservation) of plant material. Slow growth storage refers to the techniques enabling the in vitro conservation of shoot cultures in aseptic conditions by reducing markedly the frequency of periodic subculturing, without affecting the viability and regrowth of shoot cultures. Cryopreservation refers to the storage of explants from tissue culture at ultra-low temperature (-196 degrees C).

Green energy from Rhodopseudomonas palustris grown at low to high irradiance values, under fed-batch operational conditions.

Rhodopseudomonas palustris was grown under continuous irradiances of 36, 56, 75, 151, 320, 500, and 803 W m(-2), for a co-production of both bio-H(2) and biodiesel (lipids) using fed-batch conditions. The highest overall bio-H(2) produced [4.2 l(H(2)) l(culture) (-1)] was achieved at 320 W m(-2), while the highest dry biomass (3.

Cryopreservation of embryogenic callus of Aesculus hippocastanum L. by vitrification or one-step freezing.

An effective procedure for the cryopreservation of horse chestnut (Aesculus hippocastanum L.) embryogenic callus by vitrification/one-step freezing is described here. In particular, the study focused on the possibility of recovering the full proliferation potential of the embryogenic lines after storage in liquid nitrogen.